PLGF antibody (N-Term)

Catalog No. ABIN115668

Product Details anti-PLGF Antibody

Target: PLGF (PGF) (Placenta Growth Factor (PGF))

Binding Specificity: AA 19-38, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLGF antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: The antibody will react with all Human PlGF isoforms.

Purification: Affinity Chromatography on Protein A

Immunogen: N-terminal 20 amino acid peptide of native Human PIGF (Leu19 - Val38) produced in Insect Cells.

Application Details

Application Notes: Western Blot: 10 μg/mL, it will detect 25-50 ng/lane of recombinant human PlGF-1 andPlGF-2 under reducing conditions. Dimers are detected at higher protein amounts/lane. ELISA: 1-5 μg/mL, allows the detection of 1.0-2.5 ng/well of recombinant human PlGF-1 andPlGF-2. Immunocytochemistry: 5-25 μg/mL, allows the detection of PlGF protein in different humantissue including placenta and brain tumours.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.4 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details for PLGF

Target: PLGF (PGF) (Placenta Growth Factor (PGF))

Alternative Name: Placenta Growth Factor / PGF (PGF Products)

Synonyms: D12S1900 antibody, PGFL antibody, PLGF antibody, PlGF-2 antibody, SHGC-10760 antibody, AI854365 antibody, PIGF antibody, Plgf antibody, PlGF antibody, placental growth factor antibody, placenta growth factor antibody, PGF antibody, Pgf antibody, PLGF antibody

Background: Placenta growth factor (PlGF) is a member of the PDGF/VEGF family of growth factors that share a conserved pattern of eight cysteines. Alternate splicing results in at least three human mature PlGF forms containing 131 (PlGF1), 152 (PlGF2), and 203 (PlGF3) amino acids (aa) respectively. Only PlGF2 contains a highly basic heparinbinding 21 aa insert at the C-terminus. In the mouse, only one P lGF that is the equivalent of human PlGF2 has been identified. Human PlGF1 shares 56 % , 55 %, 74 % and 95 % aa identity with the appropriate isoform of mouse, rat, canine and equine PlGF. PlGF is mainly found as variably glycosylated, secreted, 55 - 60 kDa disulfide linked homodimers. Mammalian cells expressing PlGF include villous trophoblasts, decidual cells, erythroblasts, keratinocytes and some endothelial cells. Circulating PlGF increases during pregnancy, reaching a peak in mid-gestation, this increase is attenuated in preeclampsia. However, deletion of PlGF in the mouse does not affect development or reproduction. Postnatally, mice lacking PlGF show impaired angiogenesis in response to ischemia. PlGF binds and signals through VEGF R1/Flt1, but not VEGF R2/Flk-1/KDR, while VEGF binds both but signals only through the angiogenic receptor, VEGF R2. PlGF and VEGF therefore compete for binding to VEGF R1, allowing high PlGF to discourage VEGF/VEGF R1 binding and promote VEGF/VEGF R2mediated angiogenesis. However, PlGF (especially PlGF1) and some forms of VEGF can form dimers that decrease the angiogenic effect of VEGF on VEGF R2. PlGF2, but not PLGF-1, shows heparindependent binding of neuropilin (Npn)-1 and Npn2. PlGF induces monocyte activation, migration, and production of inflammatory cytokines and VEGF. These activities facilitate wound and bone fracture healing, but also contribute to inflammation in active sickle cell disease and atherosclerosis.Synonyms: PGFL, PLGF, PlGF

Gene ID: 5228

NCBI Accession: NP_001193941

UniProt: P49763

Pathways: VEGFR1 Specific Signals