IL12A antibody

Catalog No. ABIN115777

Product Details anti-IL12A Antibody

Target: IL12A (Interleukin 12 alpha (IL12A))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL12A antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificity: Detects Mouse IL-12. Other species not tested.

Purification: Affinity Chromatography on Protein G

Immunogen: Recombinant murine IL-12

Isotype: IgG1

Application Details

Application Notes: ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 3-4 μg/mL of thisantibody will detect at least 0.1 ng/well of recombinant murine IL-12 when used withbiotinylated antigen affinity purified anti-murine IL-12 as the detection antibody. Neutralisation: To yield one-half maximal inhibition (ND50) of the biological activity ofmurine IL-12 (2.0 ng/mL), a concentration of 0.16-0.24 μg/mL of this antibody is required. Western Blot: To detect mIL-12 by Western Blot analysis this antibody can be used at aconcentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mIL-12 is 1.0-2.0 ng/lane, under reducing conditionsand 0.25-0.50 ng/lane, under non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Protect from light!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C under subdued light for one month or (in aliquots) at -20 °C for longer.

Target Details for IL12A

Target: IL12A (Interleukin 12 alpha (IL12A))

Alternative Name: Interleukin-12 alpha / IL12A (IL12A Products)

Synonyms: CLMF antibody, IL-12A antibody, NFSK antibody, NKSF1 antibody, P35 antibody, CLMF2 antibody, IL-12B antibody, NKSF antibody, NKSF2 antibody, p40 antibody, Il-12b antibody, Il12p40 antibody, Il-12p40 antibody, Il12 antibody, IL-12p40 antibody, IL-12p35 antibody, Il-12a antibody, Ll12a antibody, p35 antibody, IL12p35 antibody, Il-12 p35 antibody, IL12p40 antibody, IL12 antibody, IL-12 p35 antibody, interleukin 12A antibody, interleukin 12B antibody, interleukin 12b antibody, interleukin 12a antibody, Interleukin 12 level antibody, IL12A antibody, IL12B antibody, Il12b antibody, Il12a antibody, IL12 antibody, il12a antibody

Background: Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells, costimulation of PBL proliferation, proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10, homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.Synonyms: CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL-12A, NK cell stimulatory factor chain 1, NKSF1

Gene ID: 16159

NCBI Accession: NP_032377

UniProt: P43431

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation