IL12A antibody

Catalog No. ABIN115777

This Rat Monoclonal antibody specifically detects IL12A in WB, EIA and Func. It exhibits reactivity toward Mouse.

Quick Overview for IL12A antibody (ABIN115777)

Target: IL12A (Interleukin 12 alpha (IL12A))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL12A antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-IL12A Antibody

Specificity: Detects Mouse IL-12. Other species not tested.

Purification: Affinity Chromatography on Protein G

Immunogen: Recombinant murine IL-12

Isotype: IgG1

Application Details

Application Notes: ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 3-4 μg/mL of thisantibody will detect at least 0.1 ng/well of recombinant murine IL-12 when used withbiotinylated antigen affinity purified anti-murine IL-12 as the detection antibody. Neutralisation: To yield one-half maximal inhibition (ND50) of the biological activity ofmurine IL-12 (2.0 ng/mL), a concentration of 0.16-0.24 μg/mL of this antibody is required. Western Blot: To detect mIL-12 by Western Blot analysis this antibody can be used at aconcentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mIL-12 is 1.0-2.0 ng/lane, under reducing conditionsand 0.25-0.50 ng/lane, under non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Protect from light!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C under subdued light for one month or (in aliquots) at -20 °C for longer.

Target Details for IL12A

Target: IL12A (Interleukin 12 alpha (IL12A))

Alternative Name: Interleukin-12 alpha / IL12A

Background: Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells, costimulation of PBL proliferation, proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10, homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.Synonyms: CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL-12A, NK cell stimulatory factor chain 1, NKSF1

Gene ID: 16159

NCBI Accession: NP_032377

UniProt: P43431

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation