Leptin antibody (Biotin)

Catalog No. ABIN115975

This Rabbit Polyclonal antibody specifically detects Leptin in WB and EIA. It exhibits reactivity toward Mouse.

Quick Overview for Leptin antibody (Biotin) (ABIN115975)

Target: Leptin (LEP)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Leptin antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Leptin Antibody (Biotin)

Specificity: Recognises Murine Leptin.

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant mLeptin (murine Leptin)

Application Details

Application Notes: ELISA: (Direct): To detect mLeptin by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2- 0.4 ng/well of recombinant mLeptin. (Sandwich): To detect mLeptin by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Polyclonal Anti-Murine Leptin (PP050P1, PP050P2) as acapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mLeptin. Western Blot: To detect mLeptin by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mLeptin is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for Leptin

Target: Leptin (LEP)

Alternative Name: Leptin

Background: Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype. Defects in the Leptin gene are the cause of profound obesity and type II diabetes.Synonyms: LEP, OB, OBS, Obese protein, Obesity factor

Gene ID: 16846

NCBI Accession: NP_032519

UniProt: P41160

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process