IGF1 antibody

Catalog No. ABIN116353

This Goat Polyclonal antibody specifically detects IGF1 in WB, EIA and Func. It exhibits reactivity toward Mouse.

Quick Overview for IGF1 antibody (ABIN116353)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This IGF1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-IGF1 Antibody

Specificity: Reacts with Insulin-like Growth Factor I (IGF-I).

Purification: Affinity Chromatography.

Immunogen: E.coli derived highly pure (> 98%) recombinant Murine IGF-I.

Application Details

Application Notes: Neutralization. To yield one half-maximal inhibition [ND50] of the biological activity of mIGF-I (200 ng/mL),a reconstitution of 12-14 μg/mL of this antibody is required. ELISA(Indirect): To detect mIGF-I by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant mIGF-I. (Sandwich): To detect mIGF-I by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Murine IGF-I (PP1120B) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIGF-I. Western BlotTo detect mIGF-I by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mIGF-I is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or at -20 °C for longer.

Target Details for IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: Insulin-Like Growth Factor I / IGF1

Background: Insulin is a pancreatic hormone that regulates glucose uptake and the synthesis of protein and fat. The insulin like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth promoting activity. IGF1 (Insulin Like Growth Factor I) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. IGF1 functions as an autocrine regulator of growth.Synonyms: IBP1, IGF-I, Somatomedin

Gene ID: 16000

NCBI Accession: NP_001104745

UniProt: P05017

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation