beta 2 Defensin antibody (Biotin)

Catalog No. ABIN116359

This Goat Polyclonal antibody specifically detects beta 2 Defensin in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for beta 2 Defensin antibody (Biotin) (ABIN116359)

Target: beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This beta 2 Defensin antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-beta 2 Defensin Antibody (Biotin)

Specificity: This antibody recognizes Human BD2 (Defensin beta 2). Other species not tested.

Purification: Immunoaffinity Chromatography.

Immunogen: Highly pure (>98%) recombinant Human BD-2 (hBD-2)

Application Details

Application Notes: Direct ELISA: To detect hBD-2 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hBD-2. Sandwich ELISA: To detect hBD-2 by Sandwich ELISA (using 100 μL/well antibody solution)a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with a compatible polyclonal Anti-Human BD-2 (PP1125P1 orPP1125P2) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant hBD-2. Western Blot: To detect hBD-2 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hBD-2 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at -20 °C for one year from date of despatch. The reconstituted solution can be stored at 2-8 °C for two weeks. Frozen aliquots of this solution are stable for six months when kept at -20 °C.

Expiry Date: 12 months

Target Details for beta 2 Defensin

Target: beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))

Alternative Name: Defensin beta 2

Background: This antibiotic peptide is locally regulated by inflammation. Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. hBD-2 is a antimicrobial peptide, which belongs to the distinct family of beta-defensins.Synonyms: BD-2, Beta-defensin 2, Beta-defensin 4A, DEFB102, DEFB2, DEFB4A, DEFB4B, Skin-antimicrobial peptide 1, hBD-2

Gene ID: 100289462

NCBI Accession: NP_001192195

UniProt: O15263