CDKN1B antibody

Catalog No. ABIN116519

The Rabbit Polyclonal anti-CDKN1B antibody is suitable to detect CDKN1B in samples from Human. It has been validated for WB, IP and EIA.

Quick Overview for CDKN1B antibody (ABIN116519)

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDKN1B antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-CDKN1B Antibody

Specificity: This product was Antiserum will specifically react with a p27 protein from human tissue. No reaction was observed against other related tumor suppressor proteins. No reactivity is observed with p27 from mouse. Reactivity against p27 from other species is unknown.

Purification: Prepared from monospecific antiserum by delipidation and defibrination.

Immunogen: Full length human p27 protein.

Application Details

Application Notes: Suitable for ELISA, Immunoprecipitation, Immunoblotting and other immunologicalmethods requiring high titer and specificity. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against p27 from cell lysates and E. coli produced fusion proteins at a dilution of1: 2000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG[H&L] (Goat). Expect a band at approx. 27 kDa in size corresponding to p27 by western blotting inthe appropriate cell lysate or extract. Anti-p27 is suitable for the detection by immunoblot of human, rat and mouse p27. This antisera is suitable for Immmunoprecipitation of in vitro translated protein and celllysates (C212, 3T3-L1) and will co-precipitate associated proteins.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 80 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store original vial and reconstituted antibody at 2-8 °C.

Target Details for CDKN1B

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Alternative Name: CDKN1B / KIP1

Background: P27 Kip is a cyclin-dependent kinase inhibitor that shares a limited similarity with CDK inhibitor CDKN1A/p21. p27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.Synonyms: Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1

Gene ID: 1027

NCBI Accession: NP_004055

UniProt: P46527

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy