RFX5 antibody (N-Term)

Catalog No. ABIN116534

Product Details anti-RFX5 Antibody

Target: RFX5 (Regulatory Factor X 5 (RFX5))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFX5 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Electrophoretic Mobility-Shift Assay (EMSA)

Specificity: Anti-RFX5 (N-terminal specific) may react with unknown minor bands at 85 kDa and 55 kDa. Reacts with human and mouse RFX5 from a variety of fibroblast and B-cell lysates. Recognition is at the N-terminal region. Western blot of RFX5 shows a 75 kDa band. A minor band at 68 kDa may be detected which may represent an RFX5 breakdown product or unmodified RFX5.

Purification: Prepared from monospecific antiserum by delipidation and defibrination.

Immunogen: RFX5 (N-terminal specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Application Details

Application Notes: Suitable for Immunoprecipitation, Western blotting (1/1000), ELISA and supershift assays. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against RFX5 (N-terminal specific) from a variety of fibroblast and B-cell lysates ata dilution of 1: 500 followed by reaction with Peroxidase conjugated Affinity Purifiedanti-Rabbit IgG [H&L] (Goat). Anti-RFX5 (N-terminal specific) is suitable for the detection byimmunoblot of human RFX5 a shows a 75 kDa band. This product was also tested in a gelsupershift assay and found to be reactive against RFX5 complexes using 0.5 to 1.0 l perassay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative., None

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized product at 2-8 °C. After reconstitution, store at -20 °C.

Target Details for RFX5

Target: RFX5 (Regulatory Factor X 5 (RFX5))

Alternative Name: RFX5 (RFX5 Products)

Synonyms: regulatory factor X5 antibody, regulatory factor X, 5 (influences HLA class II expression) antibody, RFX5 antibody, Rfx5 antibody

Background: RFX5 is the regulatory gene responsible for human MHC class II deficiency. Patients with this disease exhibit a characteristic defect in the binding of a nuclear factor, RFX, to the X box motif of MHC class II promoters. RFX5 is the 75 kDa subunit of the RFX complex which is absolutely essential and highly specific for regulating MHC class II gene expression and control of the immune response. RFX5 is a protein consisting of 616 amino acids.Synonyms: DNA-binding protein RFX5, Regulatory factor X 5

Gene ID: 5993

NCBI Accession: NP_000440

UniProt: P48382