Caspase 8 antibody (AA 181-478)

Catalog No. ABIN1168996

This Mouse Monoclonal antibody specifically detects Caspase 8 in WB, ICC and IP. It exhibits reactivity toward Human and has been mentioned in 8+ publications.

Quick Overview for Caspase 8 antibody (AA 181-478) (ABIN1168996)

Target: Caspase 8 (CASP8)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 8 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: C15

Product Details anti-Caspase 8 Antibody

Binding Specificity: AA 181-478

Specificity: Recognizes the p18 subunit of human caspase-8.

Cross-Reactivity: Human

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Recombinant human caspase-8 (aa 181-478).

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-Caspase 8 antibody (ABIN1168996)

Leverkus, Sprick, Wachter, Mengling, Baumann, Serfling, Bröcker, Goebeler, Neumann, Walczak: "Proteasome inhibition results in TRAIL sensitization of primary keratinocytes by removing the resistance-mediating block of effector caspase maturation." in: Molecular and cellular biology, Vol. 23, Issue 3, pp. 777-90, (2003) (PubMed).

Sprick, Rieser, Stahl, Grosse-Wilde, Weigand, Walczak: "Caspase-10 is recruited to and activated at the native TRAIL and CD95 death-inducing signalling complexes in a FADD-dependent manner but can not functionally substitute caspase-8." in: The EMBO journal, Vol. 21, Issue 17, pp. 4520-30, (2002) (PubMed).

Létuvé, Druilhe, Grandsaigne, Aubier, Pretolani: "Involvement of caspases and of mitochondria in Fas ligation-induced eosinophil apoptosis: modulation by interleukin-5 and interferon-gamma." in: Journal of leukocyte biology, Vol. 70, Issue 5, pp. 767-75, (2001) (PubMed).

Sprick, Weigand, Rieser, Rauch, Juo, Blenis, Krammer, Walczak: "FADD/MORT1 and caspase-8 are recruited to TRAIL receptors 1 and 2 and are essential for apoptosis mediated by TRAIL receptor 2." in: Immunity, Vol. 12, Issue 6, pp. 599-609, (2000) (PubMed).

Cuvillier, Mayhew, Janoff, Spiegel: "Liposomal ET-18-OCH(3) induces cytochrome c-mediated apoptosis independently of CD95 (APO-1/Fas) signaling." in: Blood, Vol. 94, Issue 10, pp. 3583-92, (1999) (PubMed).

Medema, Scaffidi, Krammer, Peter: "Bcl-xL acts downstream of caspase-8 activation by the CD95 death-inducing signaling complex." in: The Journal of biological chemistry, Vol. 273, Issue 6, pp. 3388-93, (1998) (PubMed).

Scaffidi, Fulda, Srinivasan, Friesen, Li, Tomaselli, Debatin, Krammer, Peter: "Two CD95 (APO-1/Fas) signaling pathways." in: The EMBO journal, Vol. 17, Issue 6, pp. 1675-87, (1998) (PubMed).

Scaffidi, Medema, Krammer, Peter: "FLICE is predominantly expressed as two functionally active isoforms, caspase-8/a and caspase-8/b." in: The Journal of biological chemistry, Vol. 272, Issue 43, pp. 26953-8, (1997) (PubMed).

Target Details for Caspase 8

Target: Caspase 8 (CASP8)

Alternative Name: Caspase-8

Background: Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p18, respectively, which leads to the generation of the active caspase-8 heterotetramer (p18/p10)2.

UniProt: Q14790

Pathways: Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades