Notch1 antibody

Catalog No. ABIN1169008

This Rat Monoclonal antibody specifically detects Notch1 in FACS. It exhibits reactivity toward Mouse. It has been mentioned in 2+ publications

Quick Overview for Notch1 antibody (ABIN1169008)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Notch1 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 22E5

Product Details anti-Notch1 Antibody

Purpose: anti-Notch1 (mouse), mAb (22E5)

Specificity: Recognizes endogenous mouse Notch-1 receptor.

Characteristics: Monoclonal Antibody. Recognizes endogenous mouse Notch-1 receptor. Isotype: Rat IgG2akappa. Clone: 2.20E+06. Applications: FACS. Liquid. In PBS containing 10 % glycerol and 0.02 % sodium azide. Notch signaling pathway regulates many different cell fate decisions in both vertebrate and invertebrate species. There are 5 canonical Notch ligands in mammals: Jagged-1, Jagged-2, DLL1, DLL3 and DLL4. These can bind to the four Notch receptors Notch 1-4. It is important for pattern formation during development such as neurogenesis, angiogenesis or myogenesis and regulates T cell development and stem cell maintenance. Notch signaling is also involved in cellular processes through-out adulthood. Signaling via Notch occurs between neighbouring cells and both the receptor and its ligands are transmembrane proteins.

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Recombinant mouse Notch1:Fc (AG-40B-0109).

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

+4°C

-20°C

Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-Notch1 antibody (ABIN1169008)

Ribeiro, Hasan, Wilson, Boucontet, Pereira, Lesjean-Pottier, Satoh-Takayama, Di Santo, Vosshenrich: "Cutting edge: Thymic NK cells develop independently from T cell precursors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 9, pp. 4993-7, (2010) (PubMed).

Fiorini, Merck, Wilson, Ferrero, Jiang, Koch, Auderset, Laurenti, Tacchini-Cottier, Pierres, Radtke, Luther, Macdonald: "Dynamic regulation of notch 1 and notch 2 surface expression during T cell development and activation revealed by novel monoclonal antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 11, pp. 7212-22, (2009) (PubMed).

Target Details for Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Alternative Name: Notch1

Background:

Alternate Names/Synonyms: Neurogenic Locus Notch Homolog Protein 1, Motch A, mT14, p300

Product Description: Notch signaling pathway regulates many different cell fate decisions in both vertebrate and invertebrate species. There are 5 canonical Notch ligands in mammals: Jagged-1, Jagged-2, DLL1, DLL3 and DLL4. These can bind to the four Notch receptors Notch 1-4. It is important for pattern formation during development such as neurogenesis, angiogenesis or myogenesis and regulates T cell development and stem cell maintenance. Notch signaling is also involved in cellular processes through-out adulthood. Signaling via Notch occurs between neighbouring cells and both the receptor and its ligands are transmembrane proteins.

UniProt: Q01705

Pathways: Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development