Recombinant alpha Tubulin antibody

Catalog No. ABIN1169084

There are 2+ publications for this product available. The Human Monoclonal anti-alpha Tubulin antibody is suitable to detect alpha Tubulin in samples from Human, Mouse and Cow. It has been validated for WB and ICC.

Quick Overview for Recombinant alpha Tubulin antibody (ABIN1169084)

Target: alpha Tubulin (TUBA1)

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Cow

Host: Human

Clonality: Monoclonal

Conjugate: This alpha Tubulin antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Clone: F2C

Product Details anti-alpha Tubulin Antibody

Purpose: anti-alpha-Tubulin, mAb (rec.) (F2C)

Specificity: Recognizes mouse, bovine and human alpha-tubulin.

Characteristics: Recombinant Antibody. Recognizes mouse, bovine and human alpha-tubulin. Species cross-reactivity: Bovine, Human, Mouse. Clone: F2C. Isotype: Human IgG2lambda. Applications: ICC, WB. Host: Purified from HEK 293 cell culture supernatant. Liquid. In PBS containing 10 % glycerol and 0.02 % sodium azide. Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton.

Purification: Purified from HEK 293 cell culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Bovine brain tubulin.

Isotype: IgG2 lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

anti-alpha-Tubulin, monoclonal antibody (recombinant) (F2C) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain. anti-alpha-Tubulin, monoclonal antibody (recombinant) (F2C) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

+4°C

-20°C

Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-alpha Tubulin antibody (ABIN1169084)

Vielemeyer, Nizak, Jimenez, Echard, Goud, Camonis, Rain, Perez: "Characterization of single chain antibody targets through yeast two hybrid." in: BMC biotechnology, Vol. 10, pp. 59, (2010) (PubMed).

Nizak, Martin-Lluesma, Moutel, Roux, Kreis, Goud, Perez: "Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo." in: Traffic (Copenhagen, Denmark), Vol. 4, Issue 11, pp. 739-53, (2003) (PubMed).

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: alpha-Tubulin

Background: Optional[Alternate Names/Synonyms]: Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton.

Pathways: Microtubule Dynamics