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alpha Tubulin antibody (TUBA1) Primary Antibody

TUBA1 Reactivity: Cow, Human, Mouse ICC, WB Host: Human Monoclonal F2C
Pubmed (2)
Catalog No. ABIN1169084
$517.50
Plus shipping costs $45.00
100 μg
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Delivery in 5 to 8 Business Days
  • Target
    Antibody Type
    Recombinant Antibody
    Reactivity
    Cow, Human, Mouse
    Host
    Human
    Clonality
    Monoclonal
    Conjugate
    This alpha Tubulin antibody is un-conjugated
    Application
    Immunocytochemistry (ICC), Western Blotting (WB)
    Specificity
    Recognizes mouse, bovine and human alpha-tubulin.
    Cross-Reactivity
    Cow (Bovine), Human, Mouse (Murine)
    Purification
    Purified from HEK 293 cell culture supernatant.
    Purity
    >95 % (SDS-PAGE)
    Immunogen
    Bovine brain tubulin.
    Clone
    F2C
    Isotype
    IgG2
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    anti-alpha-Tubulin, monoclonal antibody (recombinant) (F2C) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain. anti-alpha-Tubulin, monoclonal antibody (recombinant) (F2C) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    In PBS containing 10 % glycerol and 0.02 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Short Term Storage: +4°C
    Long Term Storage: -20°C
    Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.
    Expiry Date
    12 months
  • Vielemeyer, Nizak, Jimenez, Echard, Goud, Camonis, Rain, Perez: "Characterization of single chain antibody targets through yeast two hybrid." in: BMC biotechnology, Vol. 10, pp. 59, 2010 (PubMed).

    Nizak, Martin-Lluesma, Moutel, Roux, Kreis, Goud, Perez: "Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo." in: Traffic (Copenhagen, Denmark), Vol. 4, Issue 11, pp. 739-53, 2003 (PubMed).

  • Target
    Alternative Name
    alpha-Tubulin (TUBA1 Antibody Abstract)
    Synonyms
    Tuba1, K-ALPHA-1, Tuba-1, cb944, fb22g06, tuba1, wu:fb22g06, Calpha1, tubulin, alpha 1A, tubulin alpha 1b, tubulin, alpha 1b, tubulin, alpha 1B, Tuba1a, TUBA1B, tuba1b, Tuba1b
    Background
    Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton.
    Pathways
    Microtubule Dynamics
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