ZBP1 antibody (AA 1-411)

Catalog No. ABIN1169142

Product Details anti-ZBP1 Antibody

Target: ZBP1 (Z-DNA Binding Protein 1 (ZBP1))

Binding Specificity: AA 1-411

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ZBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: Recognizes human and mouse ZBP1.

Cross-Reactivity: Human, Mouse (Murine)

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Recombinant mouse ZBP1 (aa 1-411)

Clone: Zippy-1

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-ZBP1 antibody (ABIN1169142)

Christgen, Zheng, Kesavardhana, Karki, Malireddi, Banoth, Place, Briard, Sharma, Tuladhar, Samir, Burton, Kanneganti: "Identification of the PANoptosome: A Molecular Platform Triggering Pyroptosis, Apoptosis, and Necroptosis (PANoptosis)." in: Frontiers in cellular and infection microbiology, Vol. 10, pp. 237, (2020) (PubMed).

Kuriakose, Zheng, Neale, Kanneganti: "IRF1 Is a Transcriptional Regulator of ZBP1 Promoting NLRP3 Inflammasome Activation and Cell Death during Influenza Virus Infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 200, Issue 4, pp. 1489-1495, (2019) (PubMed).

Kesavardhana, Kuriakose, Guy, Samir, Malireddi, Mishra, Kanneganti: "ZBP1/DAI ubiquitination and sensing of influenza vRNPs activate programmed cell death." in: The Journal of experimental medicine, Vol. 214, Issue 8, pp. 2217-2229, (2017) (PubMed).

Target Details for ZBP1

Target: ZBP1 (Z-DNA Binding Protein 1 (ZBP1))

Alternative Name: ZBP1 (ZBP1 Products)

Synonyms: C20orf183 antibody, DAI antibody, DLM-1 antibody, DLM1 antibody, 2010010H03Rik antibody, Dai antibody, Dlm1 antibody, mZaDLM antibody, ZBP1 antibody, Z-DNA binding protein 1 antibody, ZBP1 antibody, Zbp1 antibody

Background: ZBP1 is an interferon inducible protein that binds to cytoplasmic DNA and plays a role in host defense against tumors and pathogens. ZBP1 is highly expressed in lymphatic tissues including lymph node, leukocytes, tonsil, bone marrow and spleen. ZBP1 is an innate immune sensor of Influenza A virus (IAV). ZBP1 regulates NLRP3 (nucleotide and oligomerization domain, leucine-rich repeat-containing protein family, pyrin domain containing 3) inflammasome activation as well as induction of different types of cell death (apoptosis, necroptosis and pyroptosis) during IAV infection.

UniProt: Q9H171