IDO1 antibody (N-Term)

Catalog No. ABIN3043857

Cited in 1 publication. The Rabbit Polyclonal anti-IDO1 antibody (ABIN3043857) specifically detects IDO1 in WB, IHC, IF, ICC and FACS. The antibody is reactive with Human samples.

Quick Overview for IDO1 antibody (N-Term) (ABIN3043857)

Target: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IDO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-IDO1 Antibody

Binding Specificity: N-Term

Purpose: Anti-Indoleamine 2, 3-dioxygenase/IDO1 Antibody

Sequence: NDWMFIAKHL PDLIESGQLR ERVEKLNMLS IDH

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse sequence by fourteen amino acids,and from the related rat sequence by seventeen amino acids.

Characteristics: Anti-Indoleamine 2, 3-dioxygenase/IDO1 Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human IDO1, different from the related mouse sequence by fourteen amino acids, and from the related rat sequence by seventeen amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-IDO1 antibody (ABIN3043857)

Li, Xu, Bai, Yang, Zhao, Zhang, Chen, Wang: "Umbilical Cord Tissue-Derived Mesenchymal Stem Cells Induce T Lymphocyte Apoptosis and Cell Cycle Arrest by Expression of Indoleamine 2, 3-Dioxygenase." in: Stem cells international, Vol. 2016, pp. 7495135, (2016) (PubMed).

Target Details for IDO1

Target: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Alternative Name: IDO1

Background:

Background: IDO1 (INDOLEAMINE 2,3-DIOXYGENASE), INDO or IDO, is an immunomodulatory enzyme produced by some alternatively activated macrophages and other immunoregulatory cells. This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine. By fluorescence in situ hybridization, the assignment is narrowed to chromosome 8p12-p11. INDO Interferon-gamma has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase. During inflammation, IDO is upregulated in dendritic cells and phagocytes by proinflammatory stimuli, most notably IFNG, and the enzyme then uses superoxide as a 'cofactor' for oxidative cleavage of the indole ring of tryptophan, yielding an intermediate that deformylates to L-kynurenine.

Gene Full Name: indoleamine 2,3-dioxygenase 1

Sequence Similarities: Belongs to the indoleamine 2,3-dioxygenase family.

Molecular Weight: 45 kDa

Gene ID: 3620

UniProt: P14902

Pathways: Activated T Cell Proliferation