Caspase 1 p20 antibody (Biotin)

Catalog No. ABIN1169383

This Mouse Monoclonal antibody specifically detects Caspase 1 p20 in WB, IP and IHC. It exhibits reactivity toward Mouse.It has been mentioned in 1 publication

Quick Overview for Caspase 1 p20 antibody (Biotin) (ABIN1169383)

Target: Caspase 1 p20

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 1 p20 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Clone: Casper-1

Product Details anti-Caspase 1 p20 Antibody (Biotin)

Purpose: anti-Caspase-1 (p20) (mouse), mAb (Casper-1) (Biotin)

Specificity: Recognizes endogenous full-length and activated (p20 fragment) mouse caspase-1.

Characteristics: Monoclonal Antibody. Recognizes endogenous full-length and activated (p20 fragment) mouse caspase-1. Isotype: Mouse IgG1. Clone: Casper-1. Applications: IHC, IP, WB. Liquid. In PBS containing 0.02 % sodium azide. Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1beta (IL-1beta) and IL-18 and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called Inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3 responds to a variety of agonists including crystals.

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Recombinant mouse caspase-1.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: In PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

+4°C

-20°C

Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-Caspase 1 p20 antibody (Biotin) (ABIN1169383)

Carpentier, Ni, Duggan, James, Cookson, Hamerman: "The signaling adaptor BCAP inhibits NLRP3 and NLRC4 inflammasome activation in macrophages through interactions with Flightless-1." in: Science signaling, Vol. 12, Issue 581, (2020) (PubMed).

Target Details for Caspase 1 p20

Target: Caspase 1 p20

Alternative Name: Caspase-1 p20

Background:

Alternate Names/Synonyms: Interleukin-1beta Convertase, IL-1BC, Interleukin-1beta-converting Enzyme, ICE,

Product Description: Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1beta (IL-1beta) and IL-18 and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called Inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3 responds to a variety of agonists including crystals.

UniProt: P29452