PDPK1 antibody (AA 301-312)

Catalog No. ABIN116939

The Rabbit Polyclonal anti-PDPK1 antibody is suitable to detect PDPK1 in samples from Human. It has been validated for WB.

Quick Overview for PDPK1 antibody (AA 301-312) (ABIN116939)

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDPK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PDPK1 Antibody

Binding Specificity: AA 301-312

Sequence: Peptide sequence: R-K-I-Q-E-V-W-R-Q-R-Y-Q-S

Specificity: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. This antibody is primarily directed against 60,000 MW human PDK-1 and is useful in determining its presence in various assays.

Purification: Immunoaffinity chromatography.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 301-312 of PDK-1 (genome data base accession number AC005591).

Application Details

Application Notes: This affinity purified antibody detects human PDK-1 and has been tested for use in ELISAand immunoblotting. Although untested, this reagent may be useful for Immunohistochemistry andImmunoprecipitation. It recognizes 60,000 MW human PDK-1. Reactivity in otherimmunoassays is unknown. Recommended Dilutions: This product has been assayed by immunoblot using HRPGoat-anti-Rabbit IgG [H&L] and Enhanced Chemiluminescence for detection. A workingdilution range of 1: 750 is suggested to detect PDK-1 in lysates derived from Sf9 insect cellsinfected with virus expressing myc-tagged human PDK1. In this experiment, approx. 10 μg of bothan untransfected sf9 cell lysate (not shown) and PDK-1 virus-infected Sf9 lysate wereseparated on a 10 % gel by SDS-PAGE followed by transfer to nitrocellulose. Primaryantibody reaction proceeded overnight at 4C using a 1: 750 dilution of the antibody in 1 %non-fat milk. This antibody predominantly recognizes a 60 kDa band corresponding tohuman PDK-1.

Restrictions: For Research Use only

Handling

Concentration: 1.3 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Alternative Name: PDPK1

Background: PDK-1 (3-Phosphoinositide-Dependant Protein Kinase-1) phosphorylates AGC kinases. PDK-1 activates conventional PKC and PKCz (zeta) through phosphorylation of critical threonine residues in the activation loop. PDK-1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase-3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.Synonyms: 3-phosphoinositide-dependent proteinkinase 1, PDK-1, PDK1, PDPK1, hPDK1

Gene ID: 5170

NCBI Accession: NP_002604

UniProt: O15530

Pathways: PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals