Caspase 4/11 p20 (AA 81-373) antibody (Biotin)
Reactivity: Human, Mouse
IP, WB
Host: Mouse
Monoclonal
Flamy-1
Biotin
-
- Target
- Caspase 4/11 p20
- Binding Specificity
- AA 81-373
- Reactivity
- Human, Mouse
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- Biotin
- Application
- Immunoprecipitation (IP), Western Blotting (WB)
- Specificity
- Recognizes endogenous full-length protein and activated (p20) fragment of mouse and human caspase-4/11.
- Cross-Reactivity
- Human, Mouse (Murine)
- Purification
- Purified from concentrated hybridoma tissue culture supernatant.
- Purity
- >95 % (SDS-PAGE)
- Immunogen
- Recombinant mouse Caspase-11 (aa 81-373).
- Clone
- Flamy-1
- Isotype
- IgG2b
-
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- In PBS containing 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
-
Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C. - Expiry Date
- 12 months
-
- Target
- Caspase 4/11 p20
- Background
- Inflammatory caspases (also known as group I caspases) are encoded by three main genes in humans, caspase-1, caspase-4 and caspase-5 and three main genes in mouse, caspase-1, caspase-11 and caspase-12. Caspase-11 is the murine orthologue of human caspase-4 and -5. Murine caspase-11 is a poorly characterized member of the caspase-1 subfamily. Caspase-11-deficient embryonic fibroblasts are resistant to apoptosis induced by ectopic expression of caspase-1, suggesting that caspase-11 is an upstream activator of caspase-1. Unlike caspase-1, the expression of caspase-11 is LPS-inducible and it is reasonable to postulate that other members of the family are regulated at the transcriptional or translational level by extracellular stimuli. Recently caspase-11 has been shown to be required for activation of the Nlrp3 inflammasome upon E. coli, V. cholerae and C. rodentium infection. The upstream activators of caspase-11 are TLR4 and TRIF, that modulate enteropathogen-induced inflammasome activation by promoting caspase-11 expression and activation. Formation of ASC foci (specks), a measure of NLRP3/ASC complex formation, requires caspase-11 but not caspase-1 indicating that caspase-11 acts upstream of the NLRP3/ASC complex.
- UniProt
- P70343
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