CDK9 antibody (C-Term, N-Term)

Catalog No. ABIN116944

This Rabbit Polyclonal antibody specifically detects CDK9 in WB, IF, IHC (p), IHC (fro) and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CDK9 antibody (C-Term, N-Term) (ABIN116944)

Target: CDK9 (Cyclin-Dependent Kinase 9 (CDK9))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK9 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Product Details anti-CDK9 Antibody

Binding Specificity: C-Term, N-Term

Specificity: Antiserum will specifically react with a 43 kDa CDK9 (PITALRE). Cross reactivity with CDK9 (PITALRE) from other species may also occur. No reaction was observed against other related cyclin dependent kinases.

Purification: This product was prepared from monospecific antiserum by Delipidation and Defibrination

Immunogen: Multiple synthetic peptides corresponding to C-terminal and N-terminal domains of the protein coded by the Human gene cdk9 (PITALRE). Remarks: The Murine cDNA is shown to be 98% identical with Human.

Application Details

Application Notes: This antibody has been tested for use in ELISA, Immunoprecipitation,Immunohistochemistry on Paraffin Sections and Western blot (expect a band at approx. 43 kDacorresponding to CDK9 (PITALRE) in the appropriate cell lysate or extract). Recommended Dilutions

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 75 mg/mL (by Refractometry)

Buffer: 0.02 M Potasium Phosphate, 0.12 M NaCl, pH 7.2 with 0.01 % Sodium Azide as preservative and no stabiizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and in aliquots at -80 °C. Reconstituted antibody can be stored at 2-8 °C for a limited period of time, it does not show decline in activity after two weeks at 4 °C.

Target Details for CDK9

Target: CDK9 (Cyclin-Dependent Kinase 9 (CDK9))

Alternative Name: CDK9

Background: CDK9 (PITALRE) (also known as cyclin-dependent kinase 9, Serine/threonine-protein kinase PITALRE, C-2K and Cell division cycle 2-like protein kinase 4) is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. CDK9 (PITALRE) interacts with a conserved domain in the TRAF-C region of the tumor necrosis factor signal transducer TRAF2. This kinase also was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. Tat stimulates human HIV-1 viral transcription elongation. This suggests that cyclin T1/cdk9(PITALRE) is one of the HIV-1 required host cellular cofactors generated during T cell activation. Cyclin T1/cdk9(PITALRE) is shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb. The cdk9(PITALRE) activity and cyclin T1 are essential for activation of transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev. CDK9 (PITALRE) is a ubiquitously expressed nuclear protein.Synonyms: CDC2L4, Cell division cycle 2-like protein kinase 4, Cell division protein kinase 9, Cyclin-dependent kinase 9, PITALRE, Serine/threonine-protein kinase PITALRE

Gene ID: 1025

NCBI Accession: NP_001252

UniProt: P50750

Pathways: Cell Division Cycle