SLC6A3 antibody (N-Term)

Catalog No. ABIN1169684

There are 3+ publications for this product available. The Mouse Monoclonal anti-SLC6A3 antibody is suitable to detect SLC6A3 in samples from Rat and Mouse. It has been validated for WB, ICC and IF.

Quick Overview for SLC6A3 antibody (N-Term) (ABIN1169684)

Target: SLC6A3 (Solute Carrier Family 6 (Neurotransmitter Transporter, Dopamine), Member 3 (SLC6A3))

Reactivity: Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SLC6A3 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 6V-23-23

Product Details anti-SLC6A3 Antibody

Binding Specificity: N-Term

Purpose: Dopamine Transporter Monoclonal Antibody [6V-23-23]

No Cross-Reactivity: Human

Cross-Reactivity (Details): Mouse, Rat. Does not react with Human

Purification: Protein A affinity-purified

Immunogen: Synthetic amino acid N-terminal peptide of rat dopamine transporter

Isotype: IgG1

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try at 1:1,000 (WB), 1:200 (IF)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.62 mg/mL

Buffer: Constituted in 0.02M Potassium Phosphate, 0.15M sodium chloride, 0.1 % sodium azide, pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

References for anti-SLC6A3 antibody (ABIN1169684)

Cremona, Matthies, Pau, Bowton, Speed, Lute, Anderson, Sen, Robertson, Vaughan, Rothman, Galli, Javitch, Yamamoto: "Flotillin-1 is essential for PKC-triggered endocytosis and membrane microdomain localization of DAT." in: Nature neuroscience, Vol. 14, Issue 4, pp. 469-77, (2011) (PubMed).

Foster, Vaughan: "Palmitoylation controls dopamine transporter kinetics, degradation, and protein kinase C-dependent regulation." in: The Journal of biological chemistry, Vol. 286, Issue 7, pp. 5175-86, (2011) (PubMed).

Dutta, Fei, Vaughan, Gaffaney, Wang, Lever, Reith: "Design, synthesis, and characterization of a novel, 4-[2-(diphenylmethoxy)ethyl]-1-benzyl piperidine-based, dopamine transporter photoaffinity label." in: Life sciences, Vol. 68, Issue 16, pp. 1839-49, (2001) (PubMed).

Target Details for SLC6A3

Target: SLC6A3 (Solute Carrier Family 6 (Neurotransmitter Transporter, Dopamine), Member 3 (SLC6A3))

Alternative Name: Dopamine Transporter 

Background: DA transporter antibody,DAT 1 antibody,DAT antibody,DAT1 antibody,Dopamine transporter antibody,SC6A3_HUMAN antibody

Gene ID: 13162, 24898

UniProt: Q61327, P23977

Pathways: Dopaminergic Neurogenesis