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Rabbit anti-Human IgG (Chain gamma) Antibody (FITC) - Preadsorbed

FACS, IM Polyclonal FITC

Catalog No. ABIN117262

Datasheet as PDF

Target See all IgG products

IgG
Binding Specificity
2356

502

411

188

130

123

94

29

28

28

17

9

6

6

6

6

5

2

2

1

1

1

1
Chain gamma
Reactivity
697

680

568

452

368

239

200

148

140

132

122

98

96

95

94

63

46

33

27

16

12

10

8

5

5

4

3

3

3

3

3

3

3

3

2

2

2

1

1

1

1

1
Human
Host
1727

1486

537

216

118

103

33

31

21

17

15

11

11

1
Rabbit
Clonality
3354

193

1
Polyclonal
Conjugate
587

410

408

369

297

276

228

120

99

67

66

62

62

52

48

47

44

43

43

41

39

32

32

32

31

30

29

29

28

28

28

28

28

28

28

28

28

27

26

26

25

21

19

17

17

16

16

15

15

14

10
FITC
Application
1867

1788

1785

1535

988

934

913

515

234

115

115

110

100

87

77

50

50

49

32

27

25

21

17

16

11

8

5

4

4

3

2

2

2

2

2

1

1

1

1

1
Flow Cytometry (FACS), Immunomicroscopy (IM)

Characteristics

Molar Ratio: 4.3 moles FITC per mole of Rabbit IgG

Purification

Preadsorption: Solid phase absorption

Immunogen

Human IgG gamma gamma chain
Mouse anti-Human IgG (Fab Region) antibody
ELISA, WB, FACS Monoclonal 4A11 IgG2b unconjugated

Mouse anti-Human IgG (Fc Region) antibody (PE)
ELISA, FACS Monoclonal JDC-10 IgG1 PE

Goat anti-Human IgG antibody (PE)
ELISA, FACS Polyclonal IgG PE F(ab')2 fragment

Mouse anti-Human IgG (Fc Region) antibody (APC)
ELISA, FACS Monoclonal H2 IgG2b APC

Mouse anti-Human IgG (Fab Region) antibody (PE)
FACS Monoclonal 4A11 IgG2b PE

Goat anti-Human IgG (Fc Region) antibody (PE) - Preadsorbed
FACS, FM Polyclonal IgG PE F(ab')2 fragment

Rabbit anti-Human IgG (Heavy & Light Chain) antibody (Biotin)
ELISA, IHC, WB, FACS Polyclonal IgG Biotin

Goat anti-Human IgG (Heavy Chain) antibody (TRITC)
ELISA, FACS Polyclonal IgG TRITC

Goat anti-Human IgG antibody (Biotin)
ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment

Goat anti-Human IgG (Heavy Chain) antibody (Texas Red (TR))
ELISA, FACS Polyclonal IgG Texas Red (TR)

Application Notes

Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring lot-to-lot consistency.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Comment

Absorption / Emission: 495 nm Emission Wavelength: 528 nm

Restrictions

For Research Use only
Reconstitution

Restore with 1.0 mL of deionized water (or equivalent).

Concentration

1.0 mg/mL

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01 % (w/v) sodium azide as preservative

Preservative

Sodium azide

Precaution of Use

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice

Product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.

Storage

4 °C/-20 °C

Storage Comment

Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Target

IgG

Abstract

IgG Products

Target Type

Antibody

Background

Synonyms: Human Immunoglobulin G