SAA antibody (AA 20-122) (Biotin)

Catalog No. ABIN1173882

Product Details anti-SAA Antibody (Biotin)

Target: SAA (Serum Amyloid A (SAA))

Binding Specificity: AA 20-122

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SAA antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Sequence: MGHHHHHHSG S-GG FFSFVHEAFQ GAGDMWRAYT DMKEANWKNS DKYFHARGNY DAAQRGPGGV WAAEKISDGR EAFQEFFGRG HEDTIADQEA NRHGRSGKDP NYYRPPGLPD KY

Specificity: It has been selected for its ability to recognize SAA in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: Recombinant SAA expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against SAA conjugated to biotin.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Comment:

Content: The quality control contains recombinant SAA (Gly20~Tyr122) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for SAA

Target: SAA (Serum Amyloid A (SAA))

Abstract: SAA Products

Synonyms: PIG4 antibody, SAA antibody, SAA2 antibody, TP53I4 antibody, zgc:103580 antibody, saa antibody, SAA1 antibody, Saa-1 antibody, Saa2 antibody, serum amyloid A1 antibody, serum amyloid A antibody, serum amyloid A protein antibody, serum amyloid A-3 antibody, HPS5, biogenesis of lysosomal organelles complex 2 subunit 2 antibody, serum amyloid A2 antibody, serum amyloid A 1 antibody, SAA1 antibody, saa antibody, LOC476879 antibody, SAA antibody, SAA3 antibody, HPS5 antibody, SAA2 antibody, Saa1 antibody, LOC100270725 antibody