HMGB1 antibody

Catalog No. ABIN1176834

Cited in 1 publication. The Mouse Monoclonal anti-HMGB1 antibody (Clone DPH1-1) (ABIN1176834) specifically detects HMGB1 in WB, IHC, IF, Inhibition and CA. The antibody is reactive with Mammalian samples.

Quick Overview for HMGB1 antibody (ABIN1176834)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivity: Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This HMGB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Blocking Antibody (Inhibition), Cellular Assay (CA)

Clone: DPH1-1

Product Details anti-HMGB1 Antibody

Immunogen: 17-mer peptide KGKPDAAKKGVVKAEKS

Isotype: IgG1

Application Details

Application Notes: WB: 1 µg/ml antibody in 5% milk TBS-T overnight at 4°C. Migration assay: 0.5/1/2/5 µg/ml of DPH1.1 mAb is added to 30 ng/ml of rHMGB1, according to Palumbo et al. “Extracellular HMGB1, a signal of tissue damage, induces mesoangioblast migration and proliferation”.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Handling Advice: DPH1.1 mAb is provided freeze-dried.

Storage: -20 °C

References for anti-HMGB1 antibody (ABIN1176834)

Han, Yuan, Deng, He, Zhang, Shen, Chen, Qian: "Metformin decreases LPS-induced inflammatory response in rabbit annulus fibrosus stem/progenitor cells by blocking HMGB1 release." in: Aging, Vol. 11, Issue 22, pp. 10252-10265, (2020) (PubMed).

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: High-mobility group protein B1 (HMGB1)

Background: DPH1.1 mAb recognizes all mammalian HMGB1s, including human, mouse and rat. Does not recognize HMGB2. DPH1.1 mAb can be used for Western blot (WB), immunofluorescence (IHF) and immunohistochemistry (IHC). DPH1.1 mAb blocks HMGB1-elicited cell migration in trans-well migration assays. In vivo, DPH1.1 mAb administered intravenously (220 µg/mouse) blocks recruitment of inflammatory cells to sites of necrosis and infection. The mouse monoclonal IgG1 DPH1.1 was generated by injecting C57BL/6 mice with the 17-mer peptide KGKPDAAKKGVVKAEKS. Hybridomas were produced from splenocytes by standard techniques and tested by ELISA against the immunogen and full-length HMGB1.

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome