HMGB1 antibody

Catalog No. ABIN1176834

Cited in 1 publication. The Mouse Monoclonal anti-HMGB1 antibody (Clone DPH1-1) (ABIN1176834) specifically detects HMGB1 in WB, IHC, IF, Inhibition and CA. The antibody is reactive with Human, Mouse, Rat and Mammalian samples.

Quick Overview for HMGB1 antibody (ABIN1176834)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivity: Human, Mouse, Rat, Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This HMGB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Blocking Antibody (Inhibition), Cellular Assay (CA)

Clone: DPH1-1

Product Details anti-HMGB1 Antibody

Purpose: anti HMGB1 monoclonal antibody

Sequence: KGKPDAAKKGVVKAEKS

Cross-Reactivity (Details): DPH1.1 mAb recognizes all mammalian HMGB1s, including human, mouse and rat. Does not recognize HMGB2.

Characteristics: DPH1.1 mAb recognizes all mammalian HMGB1s, including human, mouse and rat. Does not recognize HMGB2. DPH1.1 mAb can be used for Western blot (WB), immunofluorescence (IHF) and immunohistochemistry (IHC). DPH1.1 mAb blocks HMGB1-elicited cell migration in trans-well migration assays. In vivo, DPH1.1 mAb administered intravenously (220 µg/mouse) blocks recruitment of inflammatory cells to sites of necrosis and infection. The mouse monoclonal IgG1 DPH1.1 was generated by injecting C57BL/6 mice with the 17-mer peptide KGKPDAAKKGVVKAEKS. Hybridomas were produced from splenocytes by standard techniques and tested by ELISA against the immunogen and full-length HMGB1.

Immunogen: The mouse monoclonal IgG1 DPH1.1 was generated by injecting C57BL/6 mice with the 17-mer peptide P1 (KGKPDAAKKGVVKAEKS). Hybridomas were generated from splenocytes by standard techniques and tested by ELISA against the immunogen and full-length HMGB1.

Isotype: IgG1

Application Details

Application Notes: WB: 1 µg/ml antibody in 5% milk TBS-T overnight at 4°C. Migration assay: 0.5/1/2/5 µg/ml of DPH1.1 mAb is added to 30 ng/ml of rHMGB1, according to Palumbo et al. “Extracellular HMGB1, a signal of tissue damage, induces mesoangioblast migration and proliferation”.

Comment:

DPH1.1 mAb can be used for Western blot (WB).
DPH1.1 mAb blocks HMGB1-elicited cell migration in trans-well migration assays.
In vivo, DPH1.1 mAb administered intravenously (220 μg/mouse) blocks recruitment of inflammatory cells to sites of necrosis and infection.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: The lyophilized protein once reconstituted will be dissolved in a solution containing Na3PO4 50mM and NaCl 0.15M pH 7.4.

Buffer: The antibody is lyophilized from Na3PO4 50mM and NaCl 0.15M pH 7.4

Storage: -20 °C

Storage Comment: 2-8°C when lyophilized. The protein once reconstituted with water can be stored frozen (-20°C), thawed and re-frozen.

References for anti-HMGB1 antibody (ABIN1176834)

Han, Yuan, Deng, He, Zhang, Shen, Chen, Qian: "Metformin decreases LPS-induced inflammatory response in rabbit annulus fibrosus stem/progenitor cells by blocking HMGB1 release." in: Aging, Vol. 11, Issue 22, pp. 10252-10265, (2020) (PubMed).

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: High-mobility group protein B1 (HMGB1)

Background: HMGB1 is a nuclear protein that is released passively by necrotic cells, retained by apoptotic cells, and secreted actively by inflammatory cells.

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome