LCK antibody (N-Term) (Alexa Fluor 647)

Catalog No. ABIN1176916

This Mouse Monoclonal antibody specifically detects LCK in ICS. It exhibits reactivity toward Human and Mouseand has been mentioned in 1 publication.

Quick Overview for LCK antibody (N-Term) (Alexa Fluor 647) (ABIN1176916)

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This LCK antibody is conjugated to Alexa Fluor 647

Application: Intracellular Staining (ICS)

Clone: MOL 171

Product Details anti-LCK Antibody (Alexa Fluor 647)

Binding Specificity: N-Term

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human N-terminal Lck

Isotype: IgG1 kappa

Application Details

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-LCK antibody (Alexa Fluor 647) (ABIN1176916)

Moroi, Koga, Nakamura, Ohtsu, Kimura, Nomoto: "Accumulation of p60lck in HTLV-I-transformed T cell lines detected by an anti-lck monoclonal antibody, MOL 171." in: Japanese journal of cancer research : Gann, Vol. 82, Issue 8, pp. 909-15, (1991) (PubMed).

Target Details for LCK

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Alternative Name: Lck

Background: Lck is a member of the Src family of cytoplasmic protein-tyrosine kinases (PTKs) that is normally expressed exclusively in lymphoid cells, primarily T lymphocytes and NK cells. A low level of expression has been detected in B lymphocytes, but its function in B cells is unknown. Its expression in other leukocytes is not well defined. Members of the Src family have several common features: 1) unique N-terminal domains, 2) attachment to cellular membranes through a myristylated N-terminus, and 3) homologous SH2, SH3, and catalytic domains. The unique N-terminal domain of Lck interacts with the cytoplasmic tails of the CD4 and CD8 cell-surface glycoproteins of T lymphocytes, which recognize antigen presenting cells via their surface MHC class II and class I molecules, respectively. The catalytic activity of Lck is regulated by both kinases and phosphatases that control the phosphorylation states of two tyrosine residues that have opposing effects. Repression of Lck's catalytic activity occurs via phosphorylation at tyrosine 505 (Y505), located near the carboxy terminus. Phosphorylation of this tyrosine site is mediated by the Csk family of PTKs, and its dephosphorylation is mediated by the protein tyrosine phosphatase, CD45. When Lck is phosphorylated at this site, it assumes a folded tertiary structure which is enzymatically inactive. When CD45 dephosphorylates it at Y505, Lck is able to autophosphorylate its Y394, which leads to conformational changes in the catalytic domain that induce kinase activity. However, it has been observed that the inhibitory effect of the phosphorylated Y505 can be overcome by direct engagement of Lck's SH3 domain and that both Y394 and Y505 are phosphorylated together in cells activated by hydrogen peroxide. Activated Lck phosphorylates the ITAMs (Immunoreceptor-based Tyrosine Activation Motifs) of the T cell receptor (TCR) and thus is critical for activation and development of T lymphocytes. The interactions of Lck, Csk, CD45, CD4 or CD8, and TCR are only a small part of a complex immunoregulatory cascade that involves additional substrates for Csk and CD45, other enzymes, adhesion molecules, adaptor proteins, and specialized membrane microdomains. The MOL 171 monoclonal antibody recognizes the 56- and 60-kDa forms of human Lck protein, regardless of phosphorylation status. It cross reacts with mouse Lck.

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling