MEK1 antibody (pSer298) (Alexa Fluor 488)

Catalog No. ABIN1177092

Cited in 3+ publications. The Alexa Fluor 488-conjugated Mouse Monoclonal anti-MEK1 antibody (Clone J114-64) (ABIN1177092) specifically detects MEK1 in ICS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MEK1 antibody (pSer298) (Alexa Fluor 488) (ABIN1177092)

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MEK1 antibody is conjugated to Alexa Fluor 488

Application: Intracellular Staining (ICS)

Clone: J114-64

Product Details anti-MEK1 Antibody (Alexa Fluor 488)

Binding Specificity: pSer298

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human MEK1 Peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-MEK1 antibody (Alexa Fluor 488) (ABIN1177092)

Slack-Davis, Eblen, Zecevic, Boerner, Tarcsafalvi, Diaz, Marshall, Weber, Parsons, Catling: "PAK1 phosphorylation of MEK1 regulates fibronectin-stimulated MAPK activation." in: The Journal of cell biology, Vol. 162, Issue 2, pp. 281-91, (2003) (PubMed).

Eblen, Slack, Weber, Catling: "Rac-PAK signaling stimulates extracellular signal-regulated kinase (ERK) activation by regulating formation of MEK1-ERK complexes." in: Molecular and cellular biology, Vol. 22, Issue 17, pp. 6023-33, (2002) (PubMed).

Kolch: "Meaningful relationships: the regulation of the Ras/Raf/MEK/ERK pathway by protein interactions." in: The Biochemical journal, Vol. 351 Pt 2, Issue 2, pp. 289-305, (2001) (PubMed).

Target Details for MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Alternative Name: MEK1

Background: MEK (Map/Erk Kinase) 1 and 2 are serine/threonine kinases, also known as MAP kinase kinases (MAP2K1 and 2, MAPKK1 and 2, or MKK1 and 2). They activate the MAP (Mitogen-Activated Protein) kinases, also known as ERKs (Extracellular signal Regulated Kinases), which are critical kinases in multiple signal transduction pathways that regulate cell growth and differentiation. Activation of MEK 1 and 2 is dependent upon phosphorylation of serines 218 and/or 222 by activated MAP kinase kinase kinases (MAP3Ks), such as the Raf isoforms. Hormones, growth and differentiating factors, or tumor promoters induce Raf activation via activation of Ras proteins. Alternatively, cellular adhesion can lead to phosphorylation of MEK1 at serine 298 (S298), mediated by p21-activated kinase (PAK). The S298-phosphorylated MEK1 has an enhanced capacity to interact with Raf, resulting in MEK1 activation. The J114-64 monoclonal antibody recognizes the phosphorylated S298 of MEK1.
Synonyms: MAP2K1, MAPKK1, MKK1, MP2K1, PPKMK1

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling