PDPK1
Reactivity: Human
WB, ELISA
Host: Mouse
Monoclonal
2E2
unconjugated
Sample Volume
20 μL
Restrictions
For Research Use only
Format
Liquid
Buffer
Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Komander, Kular, Deak, Alessi, van Aalten: "Role of T-loop phosphorylation in PDK1 activation, stability, and substrate binding." in: The Journal of biological chemistry, Vol. 280, Issue 19, pp. 18797-802, (2005) (PubMed).
Target
PDPK1
(3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))
pdpk1 antibody, MGC82080 antibody, PDK1 antibody, PDPK2 antibody, PRO0461 antibody, Pdk1 antibody, zgc:153787 antibody, 3'-phosphoinositide-dependent protein kinase 1 antibody, 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 antibody, ATPDK1 antibody, AtPDK1 antibody, T32M21.110 antibody, T32M21_110 antibody, zgc:77318 antibody, 3-phosphoinositide dependent protein kinase 1 antibody, 3-phosphoinositide dependent protein kinase 1 L homeolog antibody, 3-phosphoinositide dependent protein kinase-1 antibody, 3-phosphoinositide dependent protein kinase 1a antibody, 3-phosphoinositide-dependent protein kinase 1 antibody, 3'-phosphoinositide-dependent protein kinase 1 antibody, 3-phosphoinositide dependent protein kinase 1b antibody, PDPK1 antibody, pdpk1.L antibody, pdpk1 antibody, Pdpk1 antibody, pdpk1a antibody, LOC100380750 antibody, pdk-1 antibody, PDK1 antibody, pdpk1b antibody
Background
The serine/threonine kinase 3-Phosphoinositide-Dependent Protein Kinase-1 (PDPK1, also known as PDK1) contributes to the activation of many important kinases in the insulin and IGF-1 signaling pathways. It acts downstream of phosphatidylinositol 3-kinase (PI3-kinase) to phosphorylate residues in the activation loops of many cellular kinases, including protein kinase B (PKB/Akt), PKC isoforms, p70 S6 kinase, and PDPK1 itself. The autophosphorylation of PDPK1 at serine 241 (S241) has recently been suggested to play a role in the regulation of PDPK1. It has been proposed that PDPK1 activity plays a key role in the regulation of various cellular events such as cell proliferation, differentiation, and apoptosis. The J666-653.44.17 monoclonal antibody recognizes the phosphorylated S241 in the activation loop of human PDPK1. The orthologous phosphorylation site in mouse and rat PDPK1 is S244. Synonyms: PDK1 (pS241), PKB Kinase (pS241)