PLK1 antibody (pThr210) (Alexa Fluor 647)

Catalog No. ABIN1177152

Cited in 1 publication. The Alexa Fluor 647-conjugated Mouse Monoclonal anti-PLK1 antibody (Clone K50-483) (ABIN1177152) specifically detects PLK1 in ICS. The antibody is reactive with Human and Mouse samples.

Quick Overview for PLK1 antibody (pThr210) (Alexa Fluor 647) (ABIN1177152)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This PLK1 antibody is conjugated to Alexa Fluor 647

Application: Intracellular Staining (ICS)

Clone: K50-483

Product Details anti-PLK1 Antibody (Alexa Fluor 647)

Binding Specificity: pThr210

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human PLK1 Peptide

Isotype: IgG1 kappa

Application Details

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

References for anti-PLK1 antibody (Alexa Fluor 647) (ABIN1177152)

Jang, Ma, Terada, Erikson: "Phosphorylation of threonine 210 and the role of serine 137 in the regulation of mammalian polo-like kinase." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 44115-20, (2002) (PubMed).

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: PLK1

Background: Polo-like kinase (PLK1) is a serine/threonine kinase with structural similarities to Drosophila's Polo kinase and the Cdc5p of Saccharomyces cerevisiae. Like its invertebrate counterparts, PLK1 activity is required for DNA synthesis and is regulated throughout the cell cycle. Furthermore, PLK1 is highly expressed in primary tumors. It associates with the mitotic spindle during mitosis suggesting that, in addition to its role during S phase, PLK1 may play a role during chromosome segregation. This is consistent with its potential role in cancer development. Threonine 210 (T210) is one of the major phoshorylation sites in activated PLK1 obtained from human mitotic cells. The K50-483 monoclonal antibody recognizes the phosphorylated T210 of human PLK1.

Pathways: Cell Division Cycle, M Phase