Retinoblastoma 1 antibody (pSer780) (Alexa Fluor 488)

Catalog No. ABIN1177158

This anti-Retinoblastoma 1 antibody is a Mouse Monoclonal antibody detecting Retinoblastoma 1 in ICS. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for Retinoblastoma 1 antibody (pSer780) (Alexa Fluor 488) (ABIN1177158)

Target: Retinoblastoma 1 (RB1)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Retinoblastoma 1 antibody is conjugated to Alexa Fluor 488

Application: Intracellular Staining (ICS)

Clone: J146-35

Product Details anti-Retinoblastoma 1 Antibody (Alexa Fluor 488)

Binding Specificity: pSer780

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human Rb

Isotype: IgG1 kappa

Application Details

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

References for anti-Retinoblastoma 1 antibody (Alexa Fluor 488) (ABIN1177158)

Knudsen, Wang: "Dual mechanisms for the inhibition of E2F binding to RB by cyclin-dependent kinase-mediated RB phosphorylation." in: Molecular and cellular biology, Vol. 17, Issue 10, pp. 5771-83, (1997) (PubMed).

Target Details for Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Alternative Name: Rb

Background: The retinoblastoma gene product (Rb) is well known as a tumor suppressor and is either absent or mutated in many human tumors. Retrovirus-mediated gene transfer of the wild-type Rb gene into several Rb mutant neoplastic cell lines suppresses their tumorgenicity. Rb is a 110-kDa nuclear phosphoprotein that undergoes differential phosphorylation during the cell cycle. During G1 phase, Rb is predominantly in a hypophosphorylated state. It becomes increasingly phosphorylated throughout the cell cycle until late mitosis, when substantial dephosphorylation occurs. Hypophosphorylated Rb interacts with a number of cellular proteins including the E2F transcription factor, several cyclins, RBP-1, RBP-2, c-Abl, c-myc, N-myc, and p46. Phosphorylation of Rb at various sites, by Cyclin-dependent protein kinases, inhibits the binding of Rb to these proteins. Rb is thought to mediate its effects, in part, via the repression of genes required for proliferation. For example, Rb is specifically recruited to promoters containing E2F sites and actively represses E2F mediated transcription. Rb also stimulates the activity of other transcription factors, although the mechanisms are less clearly defined. Thus, Rb appears to regulate transcription in its aim to control cell growth. The J146-35 monoclonal antibody recognizes Rb phosphorylated at serine 780 (pS780), which affects Rb binding to E2F. The orthologous phosphorylation sites in mouse and rat Rb are serines 773 and 751, respectively.

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy