TEK antibody (pTyr1102) (PE)

Catalog No. ABIN1177218

This anti-TEK antibody is a Mouse Monoclonal antibody detecting TEK in ICS. Suitable for Human and Mouse. This Primary Antibody has been cited in 6+ publications.

Quick Overview for TEK antibody (pTyr1102) (PE) (ABIN1177218)

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This TEK antibody is conjugated to PE

Application: Intracellular Staining (ICS)

Clone: K93-754

Product Details anti-TEK Antibody (PE)

Binding Specificity: pTyr1102

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human TIE2 Peptide

Isotype: IgG2a kappa

Application Details

Application Notes: Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-TEK antibody (PE) (ABIN1177218)

Audero, Cascone, Maniero, Napione, Arese, Lanfrancone, Bussolino: "Adaptor ShcA protein binds tyrosine kinase Tie2 receptor and regulates migration and sprouting but not survival of endothelial cells." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 13224-33, (2004) (PubMed).

DeBusk, Hallahan, Lin: "Akt is a major angiogenic mediator downstream of the Ang1/Tie2 signaling pathway." in: Experimental cell research, Vol. 298, Issue 1, pp. 167-77, (2004) (PubMed).

Peters, Kontos, Lin, Wong, Rao, Huang, Dewhirst, Sankar: "Functional significance of Tie2 signaling in the adult vasculature." in: Recent progress in hormone research, Vol. 59, pp. 51-71, (2004) (PubMed).

Jones, Iljin, Dumont, Alitalo: "Tie receptors: new modulators of angiogenic and lymphangiogenic responses." in: Nature reviews. Molecular cell biology, Vol. 2, Issue 4, pp. 257-67, (2001) (PubMed).

Murray, Padrique, Pinko, McTigue: "Mechanistic effects of autophosphorylation on receptor tyrosine kinase catalysis: enzymatic characterization of Tie2 and phospho-Tie2." in: Biochemistry, Vol. 40, Issue 34, pp. 10243-53, (2001) (PubMed).

Edgell, McDonald, Graham: "Permanent cell line expressing human factor VIII-related antigen established by hybridization." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 12, pp. 3734-7, (1983) (PubMed).

Target Details for TEK

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Alternative Name: TIE2

Background: TIE2 (Tyrosine kinase with Immunoglobulin-like and EGF-like domains 2), also known as TEK (Tunical Endothelial Kinase), is an endothelial and hematopoietic cell-specific receptor tyrosine kinase (RTK) that is critical to the development and maintenance of the vasculature and highly conserved among vertebrate species. The angiopoietins are ligands of TIE2, and the abnormal vascular growth that often occurs in solid tumors is a result of disruptions in the coordinated actions of the angiopoietins, TIE2, and the closely related TIE1 RTK. Upon activation by angiopoietins, TIE2 autophosphorylates at least 2 tyrosines in its protein kinase domain and at least 3 tyrosines in its C-terminal tail. Phosphorylation at tyrosine 1102 (Y1102) leads to activation of the Akt signal transduction pathway, enhancing endothelial cell survival. The phosphorylated Y1102 also recruits and activates the src homology 2 domain-containing adaptor proteins Grb2 and ShcA, affecting endothelial cell adhesion and motility. The K93-754 monoclonal antibody recognizes the phosphorylated Y1102 in the C-terminal tail of activated TIE2. The orthologous phosphorylation site in mouse TIE2 is Y1100.
Synonyms: TEK, VMCM, CD202b

Pathways: RTK Signaling, Growth Factor Binding