Bcl-2 antibody (pSer70) (Alexa Fluor 647)

Catalog No. ABIN1177234

This Mouse Monoclonal antibody specifically detects Bcl-2 in ICS. It exhibits reactivity toward Human and has been mentioned in 5+ publications.

Quick Overview for Bcl-2 antibody (pSer70) (Alexa Fluor 647) (ABIN1177234)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Bcl-2 antibody is conjugated to Alexa Fluor 647

Application: Intracellular Staining (ICS)

Clone: N46-467

Product Details anti-Bcl-2 Antibody (Alexa Fluor 647)

Binding Specificity: pSer70

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Peptide

Isotype: IgG1

Application Details

Sample Volume: 5 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

References for anti-Bcl-2 antibody (Alexa Fluor 647) (ABIN1177234)

Geng, Tang, Li, Yang, Choi, Kazim, Zhang: "Allyl isothiocyanate arrests cancer cells in mitosis, and mitotic arrest in turn leads to apoptosis via Bcl-2 protein phosphorylation." in: The Journal of biological chemistry, Vol. 286, Issue 37, pp. 32259-67, (2011) (PubMed).

Sarkar, Korolchuk, Renna, Imarisio, Fleming, Williams, Garcia-Arencibia, Rose, Luo, Underwood, Kroemer, OKane, Rubinsztein: "Complex inhibitory effects of nitric oxide on autophagy." in: Molecular cell, Vol. 43, Issue 1, pp. 19-32, (2011) (PubMed).

Pattingre, Bauvy, Carpentier, Levade, Levine, Codogno: "Role of JNK1-dependent Bcl-2 phosphorylation in ceramide-induced macroautophagy." in: The Journal of biological chemistry, Vol. 284, Issue 5, pp. 2719-28, (2009) (PubMed).

Deng, Xiao, Lang, Gao, Ruvolo, May: "Novel role for JNK as a stress-activated Bcl2 kinase." in: The Journal of biological chemistry, Vol. 276, Issue 26, pp. 23681-8, (2001) (PubMed).

Ling, Tornos, Perez-Soler: "Phosphorylation of Bcl-2 is a marker of M phase events and not a determinant of apoptosis." in: The Journal of biological chemistry, Vol. 273, Issue 30, pp. 18984-91, (1998) (PubMed).

Target Details for Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Alternative Name: Bcl-2

Background: The N46-467 monoclonal antibody specifically binds to Bcl-2 (pS70), ie, the Bcl-2 protein phosphorylated at the Ser70 site. Bcl-2 is a ~ 26 kDa intracellular, integral membrane protein found primarily in the nuclear envelope, endoplasmic reticulum and outer mitochondrial membrane. Bcl-2 is encoded by the BCL2 (B-cell CLL/lymphoma 2) gene and is also known as Apoptosis regulator Bcl-2. Members of the Bcl-2 family play a major role in regulating the response of cells to apoptotic signals. Bcl-2 is one of the anti-apoptotic members of the Bcl-2 family. Bcl-2 knockout mice showed pronounced lymphoid apoptosis and other apoptosis related lesions later in life. Bcl-2 is a proto-oncogene because it blocks apoptosis and provides a selective survival advantage in many cell types and thus contributes to tumorigenesis. It has been implicated in several types of cancers, such as breast, prostate, and melanoma . Bcl-2 contains multiple phosphorylation sites including Thr56, Ser70, Thr74 and Ser87. Phosphorylation of Bcl-2 Ser70 has been shown to be a mitotic marker. Phosphorylation at this site regulates Bcl-2's anti-apoptotic activity and has recently been implicated in promoting autophagy. Several studies have shown that Bcl-2 phosphorylation is caused by c-Jun N-terminal kinase (JNK).
Synonyms: BCL2, Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, PPP1R50

Pathways: MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling