IL2RG antibody

Catalog No. ABIN1177316

The Rat Monoclonal anti-IL2RG antibody has been validated for FACS and Neut. It is suitable to detect IL2RG in samples from Mouse. There are 6+ publications available.

Quick Overview for IL2RG antibody (ABIN1177316)

Target: IL2RG (Interleukin 2 Receptor, gamma (IL2RG))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL2RG antibody is un-conjugated

Application: Flow Cytometry (FACS), Neutralization (Neut)

Clone: 4G3

Product Details anti-IL2RG Antibody

Brand: BD Pharmingen™

Characteristics: Neutralization Activity: Neutralization activity may be measured with a proliferation assay using 60 pg/mL recombinant human IL-15 (Cat. No. 560254) to stimulate murine CTLL-2 cells at 2x10^4 cells/mL as indicator cells (i.e preincubation of the antibody with recombinant human IL-15 for 60 minutes).
50% Neutralization (ND50) at 0.02 - 3.0 µg/mL
> 85% Neutralization at 2-20 µg/mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Isotype: IgG2a kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL2RG antibody (ABIN1177316)

He, Malek: "The IL-2 receptor gamma c chain does not function as a subunit shared by the IL-4 and IL-13 receptors. Implication for the structure of the IL-4 receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 1, pp. 9-12, (1995) (PubMed).

He, Adkins, Furse, Malek: "Expression and function of the gamma c subunit of the IL-2, IL-4, and IL-7 receptors. Distinct interaction of gamma c in the IL-4 receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 4, pp. 1596-605, (1995) (PubMed).

Malek, Furse, Fleming, Fadell, He: "Biochemical identity and characterization of the mouse interleukin-2 receptor beta and gamma c subunits." in: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, Vol. 15, Issue 5, pp. 447-54, (1995) (PubMed).

Kondo, Takeshita, Higuchi, Nakamura, Sudo, Nishikawa, Sugamura: "Functional participation of the IL-2 receptor gamma chain in IL-7 receptor complexes." in: Science (New York, N.Y.), Vol. 263, Issue 5152, pp. 1453-4, (1994) (PubMed).

Kondo, Takeshita, Ishii, Nakamura, Watanabe, Arai, Sugamura: "Sharing of the interleukin-2 (IL-2) receptor gamma chain between receptors for IL-2 and IL-4." in: Science (New York, N.Y.), Vol. 262, Issue 5141, pp. 1874-7, (1994) (PubMed).

Gillis, Ferm, Ou, Smith: "T cell growth factor: parameters of production and a quantitative microassay for activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 120, Issue 6, pp. 2027-32, (1978) (PubMed).

Target Details for IL2RG

Target: IL2RG (Interleukin 2 Receptor, gamma (IL2RG))

Alternative Name: CD132

Background: The 4G3 antibody reacts with the common gamma subunit (gammac) shared by the IL-2, IL-4, IL-7, IL-9 and IL-15 receptors also known as CD132. This receptor is expressed constitutively at low levels on most lymphocytes, myeloid cells, embryonic thymocytes and lymphoid cell lines, but not non-lymphoid cells. The gammac receptor is a 75 - 80 kD transmembrane glycoprotein which mediates signal transducing activities of cytokine receptor complexes with which it is associated.
Synonyms: Common gamma chain, gammac

Pathways: JAK-STAT Signaling, Growth Factor Binding