IL2RG antibody (Interleukin 2 Receptor, gamma)

Details for Product anti-IL2RG Antibody No. ABIN1177316
Antigen
  • Ab2-183
  • Cd132
  • CD132
  • CIDX
  • IL-2RG
  • IMD4
  • P64
  • SCIDX
  • SCIDX1
  • [g]c
  • gamma(c)
  • gc
  • interleukin 2 receptor subunit gamma
  • interleukin 2 receptor subunit gamma L homeolog
  • interleukin 2 receptor, gamma chain
  • IL2RG
  • il2rg.L
  • Il2rg
Reactivity
Mouse (Murine)
109
67
38
2
1
Host
Rat
105
14
11
Clonality (Clone)
Monoclonal ()
Conjugate
This IL2RG antibody is un-conjugated
10
10
6
5
4
2
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Neutralization (Neut)
69
48
28
18
13
6
4
4
3
2
2
1
1
1
Options
Brand BD Pharmingen™
Clone 4G3
Isotype IgG2a, kappa
Characteristics Neutralization Activity: Neutralization activity may be measured with a proliferation assay using 60 pg/mL recombinant human IL-15 (Cat. No. 560254) to stimulate murine CTLL-2 cells at 2x10^4 cells/mL as indicator cells (i.e preincubation of the antibody with recombinant human IL-15 for 60 minutes).
50% Neutralization (ND50) at 0.02 - 3.0 µg/mL
> 85% Neutralization at 2-20 µg/mL
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Plasmids, Primers & others Plasmids, Primers & others IL2RG products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name CD132 (IL2RG Antibody Abstract)
Background The 4G3 antibody reacts with the common gamma subunit (gammac) shared by the IL-2, IL-4, IL-7, IL-9 and IL-15 receptors also known as CD132. This receptor is expressed constitutively at low levels on most lymphocytes, myeloid cells, embryonic thymocytes and lymphoid cell lines, but not non-lymphoid cells. The gammac receptor is a 75 - 80 kD transmembrane glycoprotein which mediates signal transducing activities of cytokine receptor complexes with which it is associated.
Synonyms: Common gamma chain, gammac
Pathways JAK-STAT Signaling, Growth Factor Binding
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Product cited in: He, Malek: "The IL-2 receptor gamma c chain does not function as a subunit shared by the IL-4 and IL-13 receptors. Implication for the structure of the IL-4 receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 1, pp. 9-12, 1995 (PubMed).

He, Adkins, Furse, Malek: "Expression and function of the gamma c subunit of the IL-2, IL-4, and IL-7 receptors. Distinct interaction of gamma c in the IL-4 receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 4, pp. 1596-605, 1995 (PubMed).

Malek, Furse, Fleming, Fadell, He: "Biochemical identity and characterization of the mouse interleukin-2 receptor beta and gamma c subunits." in: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, Vol. 15, Issue 5, pp. 447-54, 1995 (PubMed).

Kondo, Takeshita, Higuchi, Nakamura, Sudo, Nishikawa, Sugamura: "Functional participation of the IL-2 receptor gamma chain in IL-7 receptor complexes." in: Science (New York, N.Y.), Vol. 263, Issue 5152, pp. 1453-4, 1994 (PubMed).

Kondo, Takeshita, Ishii, Nakamura, Watanabe, Arai, Sugamura: "Sharing of the interleukin-2 (IL-2) receptor gamma chain between receptors for IL-2 and IL-4." in: Science (New York, N.Y.), Vol. 262, Issue 5141, pp. 1874-7, 1994 (PubMed).

Gillis, Ferm, Ou, Smith: "T cell growth factor: parameters of production and a quantitative microassay for activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 120, Issue 6, pp. 2027-32, 1978 (PubMed).

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