GFP antibody (Biotin)

Catalog No. ABIN117892

This Mouse Monoclonal antibody specifically detects GFP in WB, IHC (fro) and EIA. It exhibits reactivity toward Aequorea victoria.

Quick Overview for GFP antibody (Biotin) (ABIN117892)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 9F9-F9

Product Details anti-GFP Antibody (Biotin)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Mouse Serum. Reactivity is observed against wild type and recombinant forms of GFP. Reactivity is observed against recombinant Green Fluorescent Protein (recombinant GFP from Aequorea victoria) by both Western blot and ELISA. No reaction is seen against RFP.

Characteristics: Molar Ratio: 10-20 BAC molecules per Mouse IgG molecule.

Purification: Protein A chromatography.

Immunogen: Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria

Isotype: IgG2a

Application Details

Application Notes: Monclonal anti-GFP is designed to detect native GFP and GFP-containing recombinantproteins. This antibody can be used to detect GFP by ELISA (sandwich or capture) for thedirect binding of antigen. Biotin conjugated monoclonal anti-GFP used in a sandwich ELISAis well suited to titrate GFP in solution using this antibody in combination with polyclonal

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % Sodium Azide, 10 mg/mL BSA (IgG and Protease free)

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y, antibodies raised against full-length GFP should also detect YFP and other variants.Synonyms: GFP-Tag, Green fluorescent protein

Gene ID: 6100

UniProt: P42212