AKT2 antibody

Catalog No. ABIN117900

This anti-AKT2 antibody is a Rabbit Polyclonal antibody detecting AKT2 in WB. Suitable for Human, Mouse and Rat.

Quick Overview for AKT2 antibody (ABIN117900)

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKT2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AKT2 Antibody

Sequence: Synthetic peptide R-Y-D-S-L-G-L-L-E-L-D-Q-R-T (correspond ing to aa 455-468 of 481)

Specificity: This affinity purified antibody is directed against human AKT2. The antibody detects both unphosphorylated and phosphorylated forms of the protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 85 % identity to mouse and 92 % identity with rat, therefore, cross reactivity is expected.

Purification: Immunoaffinity purification.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide of human AKT2 conjugated to Keyhole Limpet Hemocyanin (KLH).

Application Details

Application Notes: This antibody was tested by ELISA and Immunoblotting and was found to be reactive withboth unphosphorylated and phosphorylated AKT2 in a lysate of HEK293 cells. Although nottested, this antibody is likely functional in Immunohistochemistry andImmunoprecipitation. Recommended Dilution(s): This product has been assayed by immunoblot against aHEK293 cell lysate and is reactive at a 1: 1,000 dilution showing a band at approximately 60

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 , with 0.01 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 2-8 °C.

Target Details for AKT2

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Alternative Name: AKT2 / PKB beta

Background: AKT2 is also known as V-AKT Murine Thymoma Viral Oncogene Homolog 2 antibody, Rac protein kinase beta antibody, PKB beta antibody or PRKBB antibody. AKT2 is an isoform of the phosphoinositidedependent serine-threonine protein kinase AKT and is enriched in insulin-responsive tissues and has been implicated in the metabolic actions of the hormone. AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2- like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins. AKT2 mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT2 to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT2 dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT2 has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation.Synonyms: Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase

Gene ID: 208

NCBI Accession: NP_001617

UniProt: P31751

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling