ERBB3 antibody (C-Term)

Catalog No. ABIN117962

Product Details anti-ERBB3 Antibody

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Binding Specificity: AA 1283-1323, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERBB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Specificity: The antibody is directed against Human HER3. It can detect HER3 from Human, Mouse and Rat sources. Reactivity of this antibody with HER3 from other species is unknown.

Purification: Protein A Chromatography followed by cross adsorption against GST and extensive dialysis against the buffer stated below.Assay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Rabbit.

Immunogen: This whole serum was prepared by repeated immunizations with a GST-HER3 fusion protein corresponding to amino acids 1283 to 1323 (40 amino acids) at the carboxy-terminus of human HER3.

Application Details

Application Notes: Anti-HER3 antibody is specifically designed for Immunoprecipitation and can be used forImmunoblotting in conjunction with a detection antibody like anti-phosphotyrosine. Reactivity in other assays is likely, but has not been determined. Recognition of HER3 isindependent of the phosphorylation status of the molecule. Human prostate cell lines(LNCap) or human breast cell line (MDA-453) are typically used as positive control sources. Recommended Dilutions: Immunoprecipitation: Use approximately 5 μL of the antibody. The Immunoprecipitation

Restrictions: For Research Use only

Handling

Concentration: 5.74 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M potassium phosphate, 0.12 M sodium chloride, pH 7.2 with 0.01 % Sodium Azide as preservative without Stabilizers.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.

Target Details for ERBB3

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Alternative Name: ERBB3 / HER3 (ERBB3 Products)

Synonyms: ErbB-3 antibody, HER3 antibody, LCCS2 antibody, MDA-BF-1 antibody, c-erbB-3 antibody, c-erbB3 antibody, erbB3-S antibody, p180-ErbB3 antibody, p45-sErbB3 antibody, p85-sErbB3 antibody, ERBB3 antibody, C76256 antibody, Erbb-3 antibody, Erbb3r antibody, Her3 antibody, nuc-ErbB3 antibody, erb-b2 receptor tyrosine kinase 3 antibody, v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) antibody, ERBB3 antibody, Erbb3 antibody

Background: The human Epidermal Growth Factor Receptor (EGFR) family is a family of receptor tyrosine kinases consisting of 4 members: EGFR, HER2, HER3 and HER4. These receptors are expressed on a wide variety of tissue types and have been implicated in many human cancers, including breast, gastric, colon and prostate. Strong expression of both HER3 and HER2 (also known as p185neu) by tumor cells can be associated with poor outcome for patients. The ligand for HER3 and HER4 is called Neu Differentiation Factor (NDF) or Heregulin. In some instances, antibodies against these receptors prove effective in combination therapy regimens for treatment of the disease. Signaling through the EGFR family involves the combinatorial dimerization of the four known family members and is mediated by at least 8 different hormones, including EGF and the heregulins. Heregulin binding and tyrosine phosphorylation by HER3 or HER4 is enhanced in the presence of HER2. Structurally the members of the EGFR family are 1210 to 1342 amino acids in length with approximately 650 amino acids of the extracellular domain being heavily glycosylated. The extracellular domains show up to 70 % sequence homology.Synonyms: ERBB-3, HER-3, Proto-oncogene-like protein c-ErbB-3, Receptor tyrosine-protein kinase erbB-3, Tyrosine kinase-type cell surface receptor HER3, c-erbB-3

Gene ID: 2065

NCBI Accession: NP_001005915

UniProt: P21860

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway