RPA2 antibody (AA 115-129)

Catalog No. ABIN118008

The Rabbit Polyclonal anti-RPA2 antibody (ABIN118008) specifically detects RPA2 in WB and EIA. The antibody is reactive with Saccharomyces cerevisiae samples.

Quick Overview for RPA2 antibody (AA 115-129) (ABIN118008)

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPA2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-RPA2 Antibody

Binding Specificity: AA 115-129

Sequence: Peptide sequence: S-S-G-K-G-Y-G-S-Q-V-A-Q-Q-F-E

Specificity: This affinity purified antibody is directed against yeast RFA2. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was purified against the phosphorylated form of the immunizing peptide and then cross adsorbed against the non-phosphorylated form of the peptide. This antibody consists of antibodies eluted from the non-phospho peptide column.

Purification: Immunoaffinity chromatography.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 115-129 (of 273) of yeast DNA Replication Factor A (RFA2). The RFA complex consists of a heterotrimer of RFA1, RFA2 and RFA3, 69, 36 and 13 kda chains respectively. Yeast RFA2 is phosphorylated at the G1/S transition and is dephosphorylated at mitosis.

Application Details

Application Notes: This affinity-purified antibody was tested for use in western blotting against RFA2containing cell lysates and by ELISA against the immunizing peptide. Reactivity in otherimmunoassays is unknown. This product was assayed by western blotting against RFA2containing cell lysates. A 1: 100 to 1: 500 dilution is recommended for this assay. Thisproduct was also assayed by ELISA against 0.1 μg of the phosphorylated andnonphosphorylated form of the immunizing peptide. A 1: 1,000 to 1: 3,000 dilution of theantibody is recommended against both forms of the peptide for this assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.12 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 2-8 °C.

Target Details for RPA2

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Alternative Name: RPA2

Background: Synonyms: REPA2, RF-A protein 2, RFA2, RP-A p32, RP-A p34, RPA32, RPA34, Replication factor A protein 2, Replication protein A, Replication protein A 32 kDa subunit, Replication protein A 34 kDa subunit

Gene ID: 6118

NCBI Accession: NP_002937

UniProt: P15927

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA