CD3 antibody (FITC)
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- Target See all CD3 Antibodies
- CD3
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Reactivity
- Mouse
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This CD3 antibody is conjugated to FITC
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Application
- Flow Cytometry (FACS)
- Specificity
- This antibody recognises the CD3 antigen which is expressed by mature T cells.
- Immunogen
- Spleen cells from immunised SD rats were fused with cells of the NSO mouse myeloma cell line.
- Clone
- KT3
- Isotype
- IgG2a
- Top Product
- Discover our top product CD3 Primary Antibody
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- Application Notes
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Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells or 100 μL wholeblood. For optimal staining incubations should be performed at RT. The Fc region ofmonoclonal antibodies may bind non-specifically to cells expressing low affinity Fcreceptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- PBS containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- CD3
- Alternative Name
- CD3 (CD3 Products)
- Synonyms
- CD3 antibody, AI504783 antibody, CD3epsilon antibody, T3e antibody, cd3-gamma antibody, cd3g antibody, cd3g-a antibody, cd3g/d antibody, cd3gd antibody, t3g antibody, CD3e molecule antibody, CD3d molecule antibody, CD3 antigen, epsilon polypeptide antibody, CD3g molecule S homeolog antibody, CD3E antibody, CD3D antibody, Cd3e antibody, cd3g.S antibody
- Background
- T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
- Gene ID
- 12501
- NCBI Accession
- NP_031674
- UniProt
- P22646
- Pathways
- TCR Signaling, Ubiquitin Proteasome Pathway
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