CD15 antibody (Biotin)

Catalog No. ABIN118585

This Mouse Monoclonal antibody specifically detects CD15 in FACS. It exhibits reactivity toward Human.

Quick Overview for CD15 antibody (Biotin) (ABIN118585)

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD15 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: TG-1

Product Details anti-CD15 Antibody (Biotin)

Purification: Ion exchange chromatography and gel filtration

Immunogen: Thymocyte glycoprotein fraction adsorbed to Con A and eluted with 0.1 M glucose

Isotype: IgM

Application Details

Application Notes: In flow cytometry (1.0 x 10^6 cells ), IFA and IHC (acetone-fixed frozen sections). May be used for enumerating mature granulocytes in peripheral blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 100 µg/mL (OD280nm)

Buffer: 0.01 M PBS, pH 7.2 containing 1 % BSA as stabilizer and 0.09 % Sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CD15

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Alternative Name: CD15

Background: The CD15 antigen is expressed on approximately 90 % human circulating granulocytes (membranes and granules), 30 to 60 % of circulating monocytes and is absent from normal lymphocytes. CD15 antigen is also expressed on Reed Sternberg cells of Hodgkin's disease, on T cell lymphomas including mycosis fungoides and on some leukemias. Outside the hematopoietic system, CD15 expression is described in certain normal and neoplastic epithelial cells and in astrocytes. At least 5 major CD15 antigens (105, 135, 165, 185, 220 kDa) are present on the surface membranes of polymorphonuclear cells. The hapten occurs also in glycolipids. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis.Synonyms: Lacto-N-Fucopentaose III, Lewis X, SSEA1, Stage-Specific Embryonic Antigen, X-Hapten