FGF2 antibody

Catalog No. ABIN118917

Product Details anti-FGF2 Antibody

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FGF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognises basic fibroblast growth factor (bFGF). The antibody binds strongly to bFGF attached to proteoglycans, but is competitively displaced by heparin. Not suitable for detecting bFGF bound to cell surface receptors.

Purification: Protein G chromatography

Immunogen: Recombinant human bFGF

Clone: MC-GF1

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry on frozen sections. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FGF2

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Alternative Name: FGF Basic / FGF2 (FGF2 Products)

Synonyms: BFGF antibody, FGF-2 antibody, FGFB antibody, HBGF-2 antibody, Fgf-2 antibody, Fgfb antibody, bFGF antibody, fibroblast growth factor 2 antibody, fibroblast growth factor 2 (basic) antibody, FGF2 antibody, Fgf2 antibody, fgf2 antibody

Background: FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.Synonyms: BFGF, FGFB, Fibroblast growth factor 2 (basic), HBGF-2, HBGF2, Heparin-binding growth factor 2

Gene ID: 2247

NCBI Accession: NP_001997

UniProt: P09038

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins