GZMB antibody (Biotin)

Catalog No. ABIN119564

The Mouse Monoclonal anti-GZMB antibody is suitable to detect GZMB in samples from Human. It has been validated for EIA.

Quick Overview for GZMB antibody (Biotin) (ABIN119564)

Target: GZMB (Granzyme B (GZMB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GZMB antibody is conjugated to Biotin

Application: Enzyme Immunoassay (EIA)

Clone: GB10

Product Details anti-GZMB Antibody (Biotin)

Specificity: This antibody recognises the serine protease Granzyme B. This antibody has been reported to function as a detection reagent in ELISA assays for soluble Granzyme B in conjunction with GB11 (SM1806P) as capture reagent.

Purification: Affinity chromatography on Protein A

Immunogen: Purified human Granzyme B. Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.001 % Thiomersal

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GZMB

Target: GZMB (Granzyme B (GZMB))

Background: Ganzyme B which is expressed within the granules of cytolytic lymphocytes (CTLs). Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target cell Granzyme B activates the cascade of caspases that finally results in cell death.Synonyms: CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Cathepsin G-like 1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2, GRB, Granzyme-2, Lymphocyte protease, SECT, T-cell serine protease 1-3E

Gene ID: 3002

NCBI Accession: NP_004122

UniProt: P10144

Pathways: Apoptosis, Caspase Cascade in Apoptosis