IL-1 beta antibody

Catalog No. ABIN119945

The Mouse Monoclonal anti-IL-1 beta antibody is suitable to detect IL-1 beta in samples from Pig. It has been validated for WB.

Quick Overview for IL-1 beta antibody (ABIN119945)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-1 beta antibody is un-conjugated

Application: Western Blotting (WB)

Clone: DF8

Product Details anti-IL-1 beta Antibody

Specificity: This antibody is specific for Interleukin-1 beta (IL-1 beta).

Purification: Affinity Chromatography on Protein G

Immunogen: Recombinant Porcine IL-1 beta.

Isotype: IgG1

Application Details

Application Notes: Western Blot: 1 μg/mL - 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.015 EU/ug

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS without stabilizer and preservative

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: Interleukin-1 beta / IL-1B

Background: Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence. IL-1 beta is principally expressed by macrophages, and plays an important immunomodulatory role in the porcine immune response.Synonyms: Catabolin, IL-1 beta, IL1 beta, IL1B, IL1F2

Gene ID: 397122

NCBI Accession: NP_999220

UniProt: P26889

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome