ITGA4 antibody (FITC)

Catalog No. ABIN119958

The FITC-conjugated Rat Monoclonal anti-ITGA4 antibody (Clone 9C10 (MFR-4-B)) (ABIN119958) specifically detects ITGA4 in FACS. The antibody is reactive with Human and Mouse samples.

Quick Overview for ITGA4 antibody (FITC) (ABIN119958)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGA4 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: 9C10 (MFR-4-B)

Product Details anti-ITGA4 Antibody (FITC)

Specificity: This antibody recognises alpha 4 integrin (CD49d). Clone 9C10 (MFR4.B) is reported to significantly inhibit binding of CD49d to CD106 but only when used in combination with clone R1-2.

Purification: Affinity chromatography on Protein G

Immunogen: Mouse LyD9 haematopoietic progenitor cells. Spleen cells from immunised Lewis rats were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Neat - 1/2, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This antibody is photosensitive and should protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Alternative Name: CD49d / ITGA4

Background: CD49d is a 150kD glycoprotein that can associate with either beta 1 integrin (CD29) or beta 7 integrin to form heterodimers CD49d/CD29 (VLA-4) and alpha4/beta7 (LPAM-1) respectively. CD49d is expressed on most lymphocytes, granulocytes, monocytes and thymocytes. The primary ligands for CD49d are CD106 (VCAM-1), fibronectin and MAdCAM-1.Synonyms: CD49 antigen-like family member D, Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4

Gene ID: 16401

NCBI Accession: NP_034706

UniProt: Q00651

Pathways: Integrin Complex