CD11b antibody

Catalog No. ABIN120412

This Mouse Monoclonal antibody specifically detects CD11b in FACS, IP, IF, IHC (fro), Func and IHC (p). It exhibits reactivity toward Rat.

Quick Overview for CD11b antibody (ABIN120412)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD11b antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: OX-42

Product Details anti-CD11b Antibody

Purification: Affinity chromatography on Protein G

Immunogen: Rat peritoneal macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: 1/50 - 1/100. Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections: 1/50 - 1/100. Immunoprecipitation. Clone OX-42 has been reported as being suitable for use on paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Functionally, clone OX-42 inhibits complement mediated rosettes. We recommend the use of ABIN182004 for use in functional studies.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b

Background: CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8 % spleen cells, 44 % bone marrow cells and less than 1 % of thymocytes and is commonly used as a microglial marker in nervous tissue.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor

Gene ID: 10116

UniProt: Q63001

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation