RASSF1 antibody (Isoform A, N-Term)

Catalog No. ABIN121164

The Mouse Monoclonal anti-RASSF1 antibody (Clone 3F3) (ABIN121164) specifically detects RASSF1 in WB, IF and EIA. The antibody is reactive with Human samples.

Quick Overview for RASSF1 antibody (Isoform A, N-Term) (ABIN121164)

Target: RASSF1 (Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RASSF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Clone: 3F3

Product Details anti-RASSF1 Antibody

Binding Specificity: AA 1-340, Isoform A, N-Term

Purification: Protein-G affinity chromatography

Immunogen: Recombinant human RASSF1A (1-340 aa), purified from E. coli

Isotype: IgG1

Application Details

Application Notes: The antibody has been tested by ELISA, immunocytochemistry analysis and Western blotting to assure specificity and reactivity. Recommended dilution range for Western blot analysis is 1:500 approx. 1:2,000 (1:1000 as starting dilution).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for RASSF1

Target: RASSF1 (Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1))

Alternative Name: RASSF1

Background: The tumor suppressor gene RASSF1A (3p21.3), human Ras association domain family 1A, is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, small intestine, colon, and peripheral blood leukocytes. RASSF1A expression is inhibited by hypermethylation of the CpG island including the promoter and early transcribed regions of the human RASSF1A gene. Hypermethylated RASSF1A promoter is observed in primary tumors. Furthermore, RASSF1A is related with cell cycle regulation, apoptosis and microtubule stability.Synonyms: NORE2A, RASSF1A, RDA32, REH3P21, Ras association domain-containing protein 1

UniProt: Q9NS23