PUMA antibody (N-Term)

Catalog No. ABIN121685

The Rabbit Polyclonal anti-PUMA antibody is suitable to detect PUMA in samples from Human and Mouse. It has been validated for WB and IHC (p).

Quick Overview for PUMA antibody (N-Term) (ABIN121685)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUMA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PUMA Antibody

Binding Specificity: N-Term

Specificity: This antibody recognises the p53 - upregulated modulator of apoptosis (PUMA), also known as Bcl-2 binding component 3.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogen: Synthetic peptide corresponding to the N-terminus of human PUMA alpha. AA Sequence: ARARQEGSSPEPVEG

Isotype: IgG

Application Details

Application Notes: Western blot: 2 μg/mL - 4 μg/mL, this antibody detects a band of approximately 23 kDa in K562 cell lysates. Immunohistochemistry on paraffin sections: 10 μg/mL, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Human breast carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name: PUMA

Background: PUMA is a proapoptotic member of the BCL-2 family. PUMA is localised to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53.Synonyms: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis

UniProt: Q9BXH1

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity