CD11b antibody

Catalog No. ABIN126001

This Mouse Monoclonal antibody specifically detects CD11b in FACS, IP and Func. It exhibits reactivity toward Human.

Quick Overview for CD11b antibody (ABIN126001)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD11b antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Clone: MEM-174

Product Details anti-CD11b Antibody

Specificity: The antibody recognizes CD11b antigen (Mac-1), a 165 kDa type Itransmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.

Characteristics: Synonyms: Integrin alpha-M, CR3A, MAC-1, MAC1, CR-3 alpha chain, Cell surface glycoprotein MAC-1subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11antigen-like family member B

Purification: Protein A affinity chromatography

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human granulocytes

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Positive control: Peripheral Blood Lymphocytes. Immunoprecipitation: Positive control: GranulocytesCD11b/CD18 transfectants, Application note: Immunoprecipitation with the antibody wassuccessfuly performed using immuno-affinity sorbents (minicolumns). The finalimmunosorbent should contain 1-5 mg of antibody/1 mLof sorbent gel (e. g. agarosebeads), It is recommended to stimulate positive control material by LPS 1-2 days before toachieve better expression of target antigen). Functional Application (antibody blocks binding of LFA-1 complex to ICAM-1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Azide free phosphate buffered saline (PBS), preserved by filter sterilization, approx. pH 7.4

Preservative: Azide free

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b

Background: CD11b (integrin aM subunit) is a 165 kDa type I transmembrane glycoprotein that non-covalently associates with integrin b2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor

Gene ID: 3684

UniProt: P11215

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation