Lamin A/C antibody

Catalog No. ABIN126715

This Mouse Monoclonal antibody specifically detects Lamin A/C in WB, IF and IHC (fro). It exhibits reactivity toward Human, Cow, Xenopus laevis and Fish.

Quick Overview for Lamin A/C antibody (ABIN126715)

Target: Lamin A/C (LMNA)

Reactivity: Human, Cow, Xenopus laevis, Fish

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lamin A/C antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: X67

Product Details anti-Lamin A/C Antibody

Specificity: The monoclonal antibody cocktail decorates the karyoskeleton, i.e. the intermediate filament equivalent of the nucleus. Polypeptides reacting: Lamin isotypes of Mr 60 - 75 kDa.

Purification: Protein A affinity chromatography

Immunogen: Nuclear pore complex-lamina fraction of Xenopus laevis (XLKE-A6 cells)

Isotype: IgG1

Application Details

Application Notes: Western blotting. Immunofluorescence microscopy. Immunohistochemistry: dilute further 1: 10 (incubation time 1 h at room temperature).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 1.0 mL distilled water.

Buffer: 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Lamin A/C

Target: Lamin A/C (LMNA)

Alternative Name: Lamin-A/C (LMNA)

Background: Nuclear Lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type Lamins and B-type Lamins. The A-type Lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. Lamin A, Lamin C and Lamin Adel 10, while the B-type Lamins include two proteins arising from two distinct genes, i.e. Lamin B1 and Lamin B2. Recent evidence has revealed that mutations in A-type Lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type Lamins coincides with cell differentiation and as A-type Lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type Lamins.Synonyms: 70 kDa Lamin, LMN1, LMNA, Lamin A, Lamin A + C, Lamin-A/C, NY-REN-32, NYREN32, Nuclear Envelope Marker, Renal carcinoma antigen NY-REN-32

Gene ID: 4000

NCBI Accession: NP_005563

UniProt: P02545

Pathways: Apoptosis, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus