JNK2 antibody (Biotin)

Catalog No. ABIN126822

This anti-JNK2 antibody is a Mouse Monoclonal antibody detecting JNK2 in WB, EIA and IHC (fro). Suitable for Human, Mouse, Rat and Dog.

Quick Overview for JNK2 antibody (Biotin) (ABIN126822)

Target: JNK2 (MAPK9) (Mitogen-Activated Protein Kinase 9 (MAPK9))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This JNK2 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 12C5

Product Details anti-JNK2 Antibody (Biotin)

Specificity: This antibody specifically interacts with the N-terminus of SAPK1α/jnk2 kinases.

Characteristics: Synonyms: JNK-55, PRKM9, MAP kinase 9, Mitogen-activated protein kinase 9, Stress-activated proteinkinase JNK2, c-Jun N-terminal kinase 2

Purification: Subsequent Thiophilic Adsorbtion and Size Exclusion Chromatography

Components: incl. positive Control

Immunogen: Synthetic peptide conjugated to KLH.

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from untreated A431 cells. ELISA: 0.05 μg/mL. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.09 % Sosium Azide, PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-80 °C

Storage Comment: Store the antibody (aliquote in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for JNK2

Target: JNK2 (MAPK9) (Mitogen-Activated Protein Kinase 9 (MAPK9))

Alternative Name: MAPK9 / JNK2

Background: Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines. The SAPK1/jnk family consists of 3 isoforms: SAPK1α/jnk2, SAPK1β/jnk3, SAPK1γ/jnk1.Synonyms: JNK-55, MAP kinase 9, Mitogen-activated protein kinase 9, PRKM9, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2

Molecular Weight: 54 kDa

Gene ID: 5601

UniProt: P45984

Pathways: MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, S100 Proteins