MEK1 antibody

Catalog No. ABIN126837

The Mouse Monoclonal anti-MEK1 antibody (Clone 9G3) (ABIN126837) specifically detects MEK1 in WB and EIA. The antibody is reactive with Human, Mouse, Rat and Dog samples.

Quick Overview for MEK1 antibody (ABIN126837)

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This MEK1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 9G3

Product Details anti-MEK1 Antibody

Specificity: This antibody specifically recognizes the activation loop of MEK1/2 independent of its phosphorylation status.

Characteristics: Synonyms: MAP2K1 - mitogen-activated protein kinase kinase 1, MAPKK1, MEK1, MKK1, PRKMK1,MAP2K2 - mitogen-activated protein kinase kinase 2, MAPKK2, MEK2, MKK2, PRKMK2

Purification: Size exclusion chromatography

Components: incl. positive Control

Immunogen: Synthetic peptide conjugated to KLH

Isotype: IgG2a

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from untreated HepG2 cells. ELISA: 0.1 μg/mL (protein ELISA).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT)

Buffer: 1 mLPBS / 0.09 % Na-azide / PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Alternative Name: MAP2K1

Background: MEK (MAP Kinase Kinase) phosphorylates the MAP Kinase on both threonine and tyrosine residues of the activation motif TEY. MEK1 and MEK2 are activated by phosphorylation of two serine residues (Ser 218/222 in MEK1 and Ser 222/226 in MEK2). These phosphorylation sites are substrates of the Raf family of kinases.Synonyms: MAP2K1 - mitogen-activated protein kinase kinase 1, MAP2K2 - mitogen-activated protein kinase kinase 2, MAPKK1, MAPKK2, MEK1, MEK2, MKK1, MKK2, PRKMK1, PRKMK2

Gene ID: 5604

UniProt: Q02750

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling