PRKACA antibody (Catalytic Subunit)

Catalog No. ABIN127012

Product Details anti-PRKACA Antibody

Target: PRKACA (Protein Kinase A, alpha (PRKACA))

Binding Specificity: Catalytic Subunit

Reactivity: Human, Mouse, Rat, Cow, Sheep, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKACA antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Specificity: Recognizes a 40 kDa PKA on SDS-PAGE immunoblots

Purification: Affinity purified

Immunogen: Synthetic peptide surrounding amino acid 11 of the catalytic subunit of PKA alpha isoform.

Application Details

Application Notes: Western blot: 0.5-4 μg/mL, Jukat cell lysate can be used as a positive control. Immunoprecipitation: 20-30 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for PRKACA

Target: PRKACA (Protein Kinase A, alpha (PRKACA))

Alternative Name: PRKACA (PRKACA Products)

Synonyms: PKACA antibody, Calpha antibody, Cs-PKA antibody, PKCA1 antibody, PKA antibody, PKCD antibody, Pkaca antibody, zgc:158799 antibody, protein kinase cAMP-activated catalytic subunit alpha antibody, protein kinase, cAMP dependent, catalytic, alpha antibody, cAMP-dependent protein kinase catalytic subunit antibody, protein kinase, cAMP-dependent, catalytic, alpha, genome duplicate a antibody, PRKACA antibody, Prkaca antibody, kin-1 antibody, prkacaa antibody

Background: Protein kinase A (PKA) also known as cAMP-dependent protein kinase, is a 40 kDa protein kinase that phosphorylates serine or threonine residues in target proteins in response to elevated levels of cAMP. The second messenger cAMP is produced in response to a wide variety of hormones & neurotransmitters and regulates cellular processes such as cell growth and differentiation, ion channel conductivity, synaptic release of neurotransmitters & gene transcription. The principle intracellular target for cAMP is PKA. Inactive PKA exists as a tetrameric protein composed of two regulatory (R) subunits and two catalytic (C) subunits. Activation occurs when two cAMP molecules bind to each R subunit which causes a conformational change that releases the active C subunits. Two major PKA isozymes, type I (PKAI) and type II (PKAII) consist of RI and RII respectively, complexed with C. Three different C subunits (alpha, beta, and gamma) and four different R subunits (RI-alpha, RI-beta, RII-alpha, and RII-beta) have been identified. It has been suggested that the complex structure of PKA may be necessary for it to accomplish such diverse functions.Synonyms: PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha

Gene ID: 5566

NCBI Accession: NP_002721

UniProt: P17612

Pathways: NF-kappaB Signaling, Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, Lipid Metabolism, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection