FOXO4 antibody (pSer193)

Catalog No. ABIN127070

The Rabbit Polyclonal anti-FOXO4 antibody is suitable to detect FOXO4 in samples from Human and Hamster. It has been validated for WB.

Quick Overview for FOXO4 antibody (pSer193) (ABIN127070)

Target: FOXO4 (Forkhead Box O4 (FOXO4))

Reactivity: Human, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FOXO4 Antibody

Binding Specificity: pSer193

Specificity: Phospho-AFX (Ser193) antibody detects only the phosphorylated Ser193 of AFX and does not react with nonphosphorylated AFX.

Purification: Peptide affinity chromatography

Application Details

Application Notes: The antibody can be used for Western blotting (1-2 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 30 % glycerol, 0.5 % BSA, and 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store (in aliquots) at -20 °C. For long term storage freeze at -70 °C.

Target Details for FOXO4

Target: FOXO4 (Forkhead Box O4 (FOXO4))

Alternative Name: FOXO4 / AFX1 / MLLT7

Background: The forkhead family of transcription factors is involved in tumorigenesis in rhabdomyosarcoma and acute leukemias. Within the family, three members (FKHR, AFX, AND FKHRL1) have sequence similarity to the nematode homolog Daf 16, recently shown to mediate signaling via a pathway involving IGFR1, P13-K and Akt. There are three potential Akt phosphorylation sites on the FKHR proteins. One site, FKHR Ser256 is found in the Forkhead domain and the other two are outside this domain. Recent studies show that phosphorylation of FKHR family members by Akt promotes cell survival. Phosphorylation regulates FKHR nuclear translocation and target gene transcription. Insulin also stimulates FKHR phosphorylation at Ser253.Synonyms: Fork head domain transcription factor AFX1, Forkhead box protein O4

Gene ID: 4303

NCBI Accession: NP_001164402

UniProt: P98177

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation