Histone H2B antibody (N-Term)

Catalog No. ABIN127254

The Rabbit Polyclonal anti-Histone H2B antibody (ABIN127254) specifically detects Histone H2B in WB and IP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Histone H2B antibody (N-Term) (ABIN127254)

Target: Histone H2B

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-Histone H2B Antibody

Binding Specificity: N-Term

Purification: Peptide affinity purified

Immunogen: Synthetic peptide corresponding to the N-terminus of human Histone H2B

Application Details

Application Notes: Western Blot: 1 μg/mL. Immunoprecipitation: 20-30 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for Histone H2B

Target: Histone H2B

Background: The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranscriptional modification including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.Synonyms: H2B Histone Family, HIST1H2B